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The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.
Excitation Laser:
Blue Laser (488 nm)
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) PerCP/Cy5.5 (blue histogram), or rat IgG2a, κ PerCP/Cy5.5 (red histogram).
CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry. Recent studies have shown CCR5 to play a role in a variety of other human diseases, ranging from infectious and inflammatory diseases to cancer.
Other Names:
CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Structure:
G-coupled receptor family 1, membrane protein, 45 kD
Distribution:
Subset of T cells, monocytes
Function:
Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1
Ligand Receptor:
MIP-1α, MIP-1ß, RANTES
Antigen References:
1. Samson M, et al. 1996. Biochemistry 35:3362. 2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161. 3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147. 4. Deng H, et al. 1996. Nature 381:661. 5. Lai J, et al. 2003. CVI. 10:1123. 6. Mañes S, et al. 2003. J. Exp. Med. 198:1381. 7. Vaday GG, et al. 2006. Prostate 66:124.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD195 (CCR5)
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) FITC (filled histogram), or rat IgG2a, κ FITC (open histogram).
PE anti-human CD195 (CCR5)
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) PE (red histogram), or rat IgG2a, κ PE (green histogram).
Alexa Fluor® 488 anti-human CD195 (CCR5)
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) Alexa Fluor® 488 (filled histogram), or rat IgG2a, κ Alexa Fluor® 488 (open histogram).
Alexa Fluor® 647 anti-human CD195 (CCR5)
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) Alexa Fluor® 647 (filled histogram), or rat IgG2a, κ Alexa Fluor® 647 (open histogram).
Alexa Fluor® 700 anti-human CD195 (CCR5)
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) Alexa Fluor® 700 (filled green histogram), or rat IgG2a, κ Alexa Fluor® 700 (open black histogram).
PerCP/Cy5.5 anti-human CD195 (CCR5)
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) PerCP/Cy5.5 (blue histogram), or rat IgG2a, κ PerCP/Cy5.5 (red histogram).
Pacific Blue™ anti-human CD195 (CCR5)
Human peripheral blood lymphocytes were stained with CD195 (clone HEK/1/85a) Pacific Blue™ (filled purple histogram), or rat IgG2a, κ Pacific Blue™ (open red histogram).
