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The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Kashiwakuma D, et al. 2010. J. Immunol. 185:2730. PubMed
Human peripheral blood lymphocytes stained with CD19 (HIB19) PE and TG2/CXCR5 PerCP/Cy5.5
CXCR5 is a chemokine receptor which binds to CXCL13. CXCR5 is expressed in B cells and T folicular helper (TFH) cells (1). CXCR5 is required for migration of B cells to the follicular compartment in the spleen, lymph nodes, Peyer's patches, and tonsils, and normal maturation of primary follicle follicular dendritic cells (2, 3). CXCR5 and CXCL13 contribute in the B cell homing to Peyer's patches (4). In addition, T cell CXCR5 is required for germinal center response of normal magnitude (5).
Other Names:
CXCR5, BLR1, CD185, MDR15, MGC117347
Structure:
GPCR, seven transmembrane receptor
Distribution:
B cells and a subset of T-helper memory cells
Function:
CXCR5/CXCL13 signaling has been implicated in the migration of B cells to the follicular compartment in the spleen, lymph nodes, Peyer’s patches, and tonsils. In addition, CXCR5 and CXCL13 contribute in the B cell homing to Peyer's patches
Ligand Receptor:
CXCL13
Antigen References:
1) Bryant LV et al, J. Immunol. 179:8180-8190 2007 2) Forster R et al, Cell 87:1037–1047 1996 3) Ansel K et al, Nature 406:309–314 2000. 4) Ebisuno Y et al, J. Immunol. 171:1642-1646 2003. 5) Haynes N et al, J. Immunol. 179:5099-5108 2007.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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PerCP/Cy5.5 anti-human CD185 (CXCR5)
Human peripheral blood lymphocytes stained with CD19 (HIB19) PE and TG2/CXCR5 PerCP/Cy5.5
