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The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
Excitation Laser:
Blue Laser (488 nm)
COA:
Application Notes:
Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13 and paraffin sections28. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101231). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101248) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP (filled histogram) or rat IgG2b PerCP isotype control (open histogram) (gated on total cell population).
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Other Names:
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR
Structure:
Integrin family, associates with integrin ß2 (CD18), 170 kD
Distribution:
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram) (gated on total cells).
Biotin anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with biotinylated CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE (gated on total cells).
FITC anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) FITC (top) or rat IgG2b, κ FITC isotype control (bottom) (gated on total viable cells).
LEAF™ Purified anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with LEAF™ purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
PE anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram) (gated on total cells).
Fixed whole mount mouse spleen was stained with Alexa Fluor® 488 F4/80 (red) (clone BM8), Brilliant Violet 421™ CD3ε (green) (clone 145-2C11), and PE CD11b (blue) (clone M1/70). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
PE/Cy5 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells stained with CD11b (clone M1/70) PE/Cy5 (filled histogram) or rat IgG2b, κ PE/Cy5 isotype control (open histogram) (gated on total cells).
Purified anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
PE/Cy7 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE/Cy7 (filled histogram) or rat IgG2b, κ PE/Cy7 isotype control (open histogram) (gated on total cells).
Alexa Fluor® 488 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 488 (filled histogram) or rat IgG2b Alexa Fluor® 488 isotype control (open histogram) (gated on total cell population).
Alexa Fluor® 647 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 647 or rat IgG2b, κ Alexa Fluor® 647 isotype control (gated on total cells).
Alexa Fluor® 700 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram) (gated on total cells).
Pacific Blue™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram) (gated on total cells).
APC/Cy7 anti-mouse/human CD11b
C57BL/6 mouse bone marrow stained with M1/70 APC/Cy7
PerCP/Cy5.5 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP/Cy5.5 (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram) (gated on total cells).
PerCP anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP (filled histogram) or rat IgG2b PerCP isotype control (open histogram) (gated on total cell population).
Brilliant Violet 421™ anti-mouse/human CD11b
BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% rat serum, stained with CD11b-BV421™ (red), B220-Alexa Fluor® 647 (blue), CD14-FITC (green) in 1% BSA and 0.1% Tween-20 in PBS. Images were acquired with an automated widefield microscope (Nikon Eclipse Ti) and a CCD camera (QImaging Retiga 2000R). Emitted light was collected through 440/40, 525/50, and 700/75 nm bandpass filters. Images provided by Ann Haberman and Christine Podolski, Yale University.
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Brilliant Violet 570™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 570™ (filled histogram) or rat IgG2b, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Brilliant Violet 605™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 605™. Data shown was gated on myeloid cell population.
Brilliant Violet 650™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 650™. Data shown was gated on myeloid cell population.
Brilliant Violet 711™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 711™. Data shown was gated on the myeloid cell population.
Brilliant Violet 510™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 510™. Data shown was gated on the myeloid cell population.
Ultra-LEAF™ Purified anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with Ultra-LEAF™ purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
