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PE Streptavidin
PE Streptavidin
405203 100 µg $45.00     
405204 500 µg $160.00     
Product Details
 
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PE under optimal conditions. The solution is free of unconjugated PE.
Concentration: 0.2 mg/ml
Storage & Handling: The streptavidin-PE solution should be stored undiluted at 4°C, and protected from exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested
Recommended Usage:

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation
Laser: 		Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Enter Lot#:   
Application
Notes:

Streptavidin-Phycoerythrin (PE) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application
References:

  Publication Library
PE Streptavidin Image 1
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE


Compare all formats

See PE spectral data



Description:

Streptavidin binds to biotin with high affinity. Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm.
Other Names: Streptavidin-Phycoerythrin, SAv-PE
GeneID: NA
Latest Publications: View the latest Streptavidin articles on HighwirePress.com
UniProt: View information about Streptavidin on UniProt.org
Keywords: PE Streptavidin, PE, Streptavidin-Phycoerythrin, SAv-PE, Mouse, Human, Rat, Flow Cytometry, Intracellular Staining for Flow Cytometry, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Cell Staining BufferFC, ICC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • LEGEND MAX™ Human Mesothelin ELISA Kit with Pre-coated Plates




  • Zombie Yellow™ Fixable Viability Kit
    One day old C57BL/6 mouse

    One day old C57BL/6 mouse splenocytes were stained with Zombie Yellow™ and analyzed before fixation (purple) or after fixation and permeabilization (red). Cells alone, without Zombie Yellow™ staining, are indicated in black.

  • LEGEND MAX™ Human Soluble Nogo-B ELISA Kit with Pre-coated Plates




  • Mouse IL-17A/F ELISA MAX™ Deluxe




  • Recombinant Human IL-5




  • FITC Streptavidin
    C57BL/6 splenocytes stained with 145-2C11

    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC

  • PE Streptavidin
    C57BL/6 splenocytes stained with 145-2C11

    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE

  • PE/Cy5 Streptavidin
    C57BL/6 splenocytes stained with 145-2C11

    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-PE/Cy5

  • Recombinant Human CCL17 (TARC) (carrier-free)
    Baf3-hCCR4 cells chemoattracted by human

    Baf3-hCCR4 cells chemoattracted by human CCL17.

  • Human IFN-γ ELISA MAX™ Standard




  • Human IFN-γ ELISA MAX™ Deluxe




  • Human TNF-α ELISA MAX™ Standard




  • Human TNF-α ELISA MAX™ Deluxe




  • Human IL-4 ELISA MAX™ Standard




  • Human IL-4 ELISA MAX™ Deluxe




  • Human IL-5 ELISA MAX™ Standard




  • Human IL-5 ELISA MAX™ Deluxe




  • Human IL-6 ELISA MAX™ Standard




  • Human IL-6 ELISA MAX™ Deluxe




  • Human IL-10 ELISA MAX™ Standard




  • Human IL-10 ELISA MAX™ Deluxe




  • Human IL-12/IL-23 (p40) ELISA MAX™ Standard




  • Human IL-12/IL-23 (p40) ELISA MAX™ Deluxe




  • Mouse IFN-γ ELISA MAX™ Standard




  • Mouse IFN-γ ELISA MAX™ Deluxe




  • Mouse TNF-α ELISA MAX™ Standard




  • Mouse TNF-α ELISA MAX™ Deluxe




  • Mouse IL-2 ELISA MAX™ Standard




  • Mouse IL-2 ELISA MAX™ Deluxe




  • Mouse IL-4 ELISA MAX™ Standard




  • Mouse IL-4 ELISA MAX™ Deluxe




  • Mouse IL-5 ELISA MAX™ Standard




  • Mouse IL-5 ELISA MAX™ Deluxe




  • Mouse IL-6 ELISA MAX™ Standard




  • Mouse IL-6 ELISA MAX™ Deluxe




  • Mouse IL-12/IL-23 (p40) ELISA MAX™ Standard




  • Mouse IL-12/IL-23 (p40) ELISA MAX™ Deluxe




  • Recombinant Human Paxillin
    10ng of Paxillin recombinant protein

    10ng of Paxillin recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Paxillin antibody (Clone PAX227). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and chemiluminescence detection.

  • LEGEND MAX™ Human Latent TGF-β ELISA Kit with Pre-coated Plates




  • Human IL-12 (p70) ELISA MAX™ Standard




  • Human IL-12 (p70) ELISA MAX™ Deluxe




  • Human IL-2 ELISA MAX™ Standard




  • Human IL-2 ELISA MAX™ Deluxe




  • Human GM-CSF ELISA MAX™ Standard




  • Human GM-CSF ELISA MAX™ Deluxe




  • Mouse IL-3 ELISA MAX™ Standard




  • Mouse IL-3 ELISA MAX™ Deluxe




  • Mouse GM-CSF ELISA MAX™ Standard




  • Mouse GM-CSF ELISA MAX™ Deluxe




  • Mouse IL-1β ELISA MAX™ Standard




  • Mouse IL-1β ELISA MAX™ Deluxe




  • LEGEND MAX™ Mouse TSLP ELISA Kit with Pre-coated Plates




  • Mouse IgE ELISA MAX™ Standard




  • Mouse IgE ELISA MAX™ Deluxe




  • Mouse IL-17A ELISA MAX™ Standard




  • Mouse IL-17A ELISA MAX™ Deluxe




  • Mouse MCP-1 ELISA MAX™ Standard




  • Mouse MCP-1 ELISA MAX™ Deluxe




  • Pacific Blue™ Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, stimulated (4 hours) with (bottom) or without (top) LEAF™ purified anti-CD95, clone EOS9.1, then stained with Annexin V Pacific Blue™ and viability dye 7-AAD





  • Recombinant Human TNF-α (carrier-free)
    Human TNF-α cytotoxicity on L929

    Human TNF-α cytotoxicity on L929 cells.

  • PerCP/Cy5.5 Streptavidin
    C57BL/6 splenocytes stained with biotinylated

    C57BL/6 splenocytes stained with biotinylated 145-2C11, followed by Sav-PerCP/Cy5.5

  • Recombinant Human FGF-basic/145aa (carrier-free)
    3NIH/3T3 cell proliferation induced by

    3NIH/3T3 cell proliferation induced by human FGFb.

  • Recombinant Mouse IL-6 (carrier-free)
    7TD1 cell proliferation induced by

    7TD1 cell proliferation induced by mIL-6.

  • Recombinant Mouse GM-CSF (carrier-free)
    FDC-P1 cell proliferation induced by

    FDC-P1 cell proliferation induced by mouse GM-CSF.

  • Recombinant Mouse TNF-α (carrier-free)
    Cytotoxic effect on L929 mouse

    Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D.

  • Mouse IL-1α ELISA MAX™ Deluxe




  • Mouse IL-1α ELISA MAX™ Standard




  • Recombinant Mouse IL-2 (carrier-free)
    HT-2 cell proliferation induced by

    HT-2 cell proliferation induced by mouse IL-2.

  • Recombinant Human IL-17A (carrier-free)
    Induction of IL-6 in human

    Induction of IL-6 in human dermal fibroblast by IL-17A.

  • Recombinant Mouse IL-23 (carrier-free)
    Induction of mouse IL-17A in

    Induction of mouse IL-17A in splenocytes by mouse IL-23

  • LEGEND MAX™ Human IL-21 ELISA Kit with Pre-coated Plates




  • Mouse IL-9 ELISA MAX™ Deluxe




  • Human IL-32α ELISA MAX™ Deluxe




  • Recombinant Mouse IL-17A (carrier-free)
    Mouse IL-6 induced by mIL-17A

    Mouse IL-6 induced by mIL-17A in fetal mouse skin fibroblasts.

  • FITC Annexin V
    Human T leukemia cell line,

    Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)





  • Alexa Fluor® 647 Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was stimulated (4 hours) with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V Alexa Fluor® 647.

  • LEGEND MAX™ Human anti-mouse Ig (HAMA) ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Rat TNF-α ELISA Kit with Pre-coated Plates




  • Human IL-21 ELISA MAX™ Deluxe




  • Recombinant Mouse IL-4 (carrier-free)
    CTLL-2 proliferation induced by mouse

    CTLL-2 proliferation induced by mouse IL-4

  • Mouse IL-12 (p70) ELISA MAX™ Deluxe




  • Mouse IL-23 (p19/p40) ELISA MAX™ Deluxe




  • Mouse IL-25 (IL-17E) ELISA MAX™ Deluxe




  • LEGEND MAX™ Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human Cystatin C ELISA Kit with Pre-coated Plates




  • Recombinant Human IL-10 (mammalian expressed, carrier-free)
    Human IL-10 inhibits the production

    Human IL-10 inhibits the production of IFNγ in PMA activated PBMC.

  • LEGEND MAX™ Total TGF-β1 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Free Active TGF-β1 ELISA Kit with Pre-coated Plates




  • Mouse TSLP ELISA MAX™ Deluxe




  • Human IL-1β ELISA MAX™ Deluxe




  • LEGEND MAX™ Human Soluble CD137/4-1BB ELISA Kit with Pre-coated Plates




  • Recombinant Mouse IL-6 (ELISA Std.)




  • Recombinant Rat IL-2 (carrier-free)
    CTLL-2 proliferation induced by rat

    CTLL-2 proliferation induced by rat IL-2

  • LEGEND MAX™ Human MCP-1/CCL2 ELISA Kit with Pre-coated Plates




  • Recombinant Mouse IL-33 (carrier-free)
    IL-5 induction by mouse IL-33

    IL-5 induction by mouse IL-33 in splenocytes activated by anti-CD3 and anti-CD28. Data kindly provided Dr. Foo Y. Liew.

  • Recombinant Mouse IL-13 (carrier-free)
    TF-1 cells proliferation induced by

    TF-1 cells proliferation induced by mouse IL-13.

  • Human IL-8 ELISA MAX™ Deluxe




  • Human IL-9 ELISA MAX™ Deluxe




  • Human FGF-basic ELISA MAX™ Deluxe




  • Human IL-8 ELISA MAX™ Standard




  • Human IL-15 ELISA MAX™ Deluxe




  • Human TSLP ELISA MAX™ Deluxe




  • Recombinant Human IL-10 (carrier-free)
    MC/9 cell proliferation induced by

    MC/9 cell proliferation induced by hIL-10.

  • Human IL-1α ELISA MAX™ Deluxe




  • Human IL-22 ELISA MAX™ Deluxe




  • LEGEND MAX™ Mouse TNF-α ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-21 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-2 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human TSLP ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-6 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IFN-γ ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse GM-CSF ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-17A ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-2 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-23 (p19/p40) ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IFN-γ ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human TNF-α ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-6 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-10 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-8 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-9 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-29 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human GM-CSF ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-27 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-28A ELISA Kit with Pre-coated Plates




  • Recombinant Human TGF-β1 (carrier-free)
    TGF-β1 inhibits the proliferation of

    TGF-β1 inhibits the proliferation of HT-2 cells induced by IL-4.

  • LEGEND MAX™ Human IL-1α ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-4 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-15 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human FGF-basic ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-12 p70 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-4 ELISA Kit with Pre-coated Plates




  • Recombinant Human IL-17A/F Heterodimer (carrier-free)
    Induction of IL-6 in human

    Induction of IL-6 in human skin fibroblast by IL-17A/IL-17F heterodimer.

  • LEGEND MAX™ Human IL-22 ELISA Kit with Pre-coated Plates




  • Recombinant Rat TNF-α (carrier-free)
    Rat TNF-α cytotoxicity on L929

    Rat TNF-α cytotoxicity on L929 cells

  • Recombinant Human CCL5 (RANTES) (carrier-free)
    Chemotactic activity of human RANTES.

    Chemotactic activity of human RANTES.

  • Recombinant Mouse IL-17A/F Heterodimer (carrier-free)
    IL-6 induced by mIL-17A/IL-17F on

    IL-6 induced by mIL-17A/IL-17F on fetal mouse skin fibroblasts.

  • Recombinant Mouse VEGF-120 (carrier-free)
    VegfA induces the proliferation of

    VegfA induces the proliferation of HUVEC cells

  • Recombinant Human PDGF-BB (carrier-free)




  • Recombinant Human CCL2 (MCP-1) (carrier-free)
    THP-1 cell chemotaxis induced by

    THP-1 cell chemotaxis induced by human MCP-1.

  • Recombinant Mouse M-CSF (carrier-free)
    M-NFS60 cell proliferation induced by

    M-NFS60 cell proliferation induced by mouse M-CSF.

  • Recombinant Human CXCL12 (SDF-1α) (carrier-free)
    Bioassay for human SDF-1

    Bioassay for human SDF-1

  • Recombinant Mouse IL-3 (carrier-free)
    Bioactivity of mouse IL-3 was

    Bioactivity of mouse IL-3 was tested by a proliferation assay using M-NFS-60 cells.

  • Recombinant Human GM-CSF (carrier-free)
    TF-1 cell proliferation induced by

    TF-1 cell proliferation induced by human GM-CSF.

  • Recombinant Mouse SCF (carrier-free)
    TF-1 cell proliferation induced by

    TF-1 cell proliferation induced by mouse SCF

  • Recombinant Human IL-17F (carrier-free)
    CXCL1 induction in human fibroblast

    CXCL1 induction in human fibroblast by human IL-17F

  • LEGEND MAX™ Mouse IL-34 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-17F ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-33 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-22 ELISA Kit with Pre-coated Plates




  • Human TruStain FcX™ (Fc Receptor Blocking Solution)
    Human TruStain FcX™ treated (blue)

    Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant mouse IgG1 PE mAb (above). Dotted histogram represents unstained cells.

    Human TruStain FcX™ treated (filled

    Human TruStain FcX™ treated (filled histograms) or non-treated (open histograms) THP-1 cells stained with anti-human HLA-DR PE (red) or an isotype control (IgG2a PE, blue) mAbs. Note: non-treated cells show false-negative HLA-DR staining due to high binding of mouse IgG1 isotype.

  • Recombinant Mouse IL-12 (p70) (carrier-free)
    Mouse splenocytes IFNγ production induced

    Mouse splenocytes IFNγ production induced by mouse IL-12.

  • Recombinant Mouse IL-17F (carrier-free)
    CXCL1 induced by mouse IL-17F

    CXCL1 induced by mouse IL-17F in fetal mouse skin fibroblasts

  • Recombinant Human IL-12 (p70) (carrier-free)
    IFNγ induced by human IL-12

    IFNγ induced by human IL-12 in PBMC activated with PHA

  • Recombinant Mouse IL-25 (IL-17E) (carrier-free)
    Mouse IL-25 binding to recombinant

    Mouse IL-25 binding to recombinant IL17BR/Fc Chimera detected by functional ELISA

  • LEGEND MAX™ Human MRP8/14 (Calprotectin) ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-12 (p70) ELISA Kit with Pre-coated Plates




  • Recombinant Mouse IL-15 (carrier-free)
    CTLL-2 cell proliferation induced by

    CTLL-2 cell proliferation induced by mouse IL-15

  • Recombinant Human IL-4 (carrier-free)
    TF-1 cell proliferation induced by

    TF-1 cell proliferation induced by human IL-4

  • Recombinant Human IL-23 (carrier-free)
    Mouse splenocytes IL-17A secretion induced

    Mouse splenocytes IL-17A secretion induced by human IL-23. Bioassay activity for human IL-23.

  • Recombinant Human IL-8 (carrier-free)
    Human IL-8 bioactivity

    Human IL-8 bioactivity

  • Recombinant Human IL-2 (carrier-free)
    CTLL-2 cell proliferation induced by

    CTLL-2 cell proliferation induced by human IL-2.

  • LEGEND MAX™ Human FGL2 ELISA Kit with Pre-coated Plates




  • Recombinant Mouse IL-27 (carrier-free)
    Inhibition of IL-2 production induced

    Inhibition of IL-2 production induced by mouse IL-27 in mouse activated splenocytes.

  • Recombinant Mouse IL-12 p40 Homodimer (carrier-free)
    Mouse IL-12 p40 homodimer is

    Mouse IL-12 p40 homodimer is able to inhibit IL-12-dependent IFNγ production.

  • LEGEND MAX™ Mouse IL-17F ELISA Kit with Pre-coated Plates




  • Mouse IL-22 ELISA MAX™ Deluxe




  • LEGEND MAX™ Mouse IL-33 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-12/23 (p40) ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-13 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Rat IL-6 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-31 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human IL-23 (p19/p40) ELISA Kit with Pre-coated Plates




  • Recombinant Human IL-27 (carrier-free)
    Inhibition of IL-2 production by

    Inhibition of IL-2 production by human IL-27 in activated splenocytes

  • Recombinant Mouse IL-34 (carrier free)
    M-NSF-60 cell proliferation induced by

    M-NSF-60 cell proliferation induced by mouse IL-34.

  • LEGEND MAX™ Human IL-1β ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IL-17A/F ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates




  • Rat TNF-α ELISA MAX™ Deluxe




  • LEGEND MAX™ Human LAP ELISA Kit with Pre-coated Plates




  • Rat IL-17A ELISA MAX™ Deluxe




  • LEGEND MAX™ Rat IL-17A ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse Lactadherin ELISA Kit with Pre-coated Plates




  • Brilliant Violet 421™ Streptavidin
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.

  • Recombinant Mouse TACI-Fc Chimera (carrier-free)
    Inhibition of BAFF mediated B

    Inhibition of BAFF mediated B cell proliferation by mouse TACI-Fc.

  • Recombinant Mouse IL-7 (carrier-free)
    PHA activated PBL proliferation induced

    PHA activated PBL proliferation induced by mouse IL-7.

  • LEGEND MAX™ Mouse FGL2 ELISA Kit with Pre-coated Plates




  • Recombinant Human IL-34 (carrier free)
    Monocyte cell proliferation induced by

    Monocyte cell proliferation induced by human IL-34.

  • RBC Lysis/Fixation Solution (10X)
    Human peripheral blood cells were

    Human peripheral blood cells were stained with anti-human CD3 PerCP/Cy5.5 and anti-human CD4 FITC; red blood cells were lysed with BioLegend RBC Lysis/Fixation Solution.





  • Recombinant Mouse CXCL9 (MIG) (carrier-free)
    T cells chemoattracted by mouse

    T cells chemoattracted by mouse CXCL9.

  • Brilliant Violet 570™ Streptavidin
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 570™.

  • LEGEND MAX™ Rat IFN-γ ELISA Kit with Pre-coated Plates




  • Recombinant Human CXCL9 (MIG) (carrier-free)
    T cells chemoattracted by human

    T cells chemoattracted by human CXCL9.

  • Recombinant Human IL-3 (carrier-free)
    TF-1 cell proliferation induced by

    TF-1 cell proliferation induced by human IL-3.

  • Human Mesothelin ELISA MAX™ Deluxe




  • Recombinant Human CXCL1 (GRO-α) (carrier-free)
    Neutrophils chemoattracted by human CXCL1.

    Neutrophils chemoattracted by human CXCL1.

  • Human IL-17A ELISA MAX™ Deluxe




  • LEGEND MAX™ Human IL-17A ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Human Active MIF ELISA Kit with Pre-coated Plates




  • Recombinant Mouse IL-21 (carrier-free)
    CTLL-2 cell proliferation induced by

    CTLL-2 cell proliferation induced by mouse IL-21.

  • LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates




  • Recombinant Mouse CXCL5 (LIX) (carrier-free)
    Human neutrophils chemoattracted by mouse

    Human neutrophils chemoattracted by mouse CXCL5.

  • Recombinant Human CXCL5 (ENA-78) (carrier-free)
    Neutrophils chemoattracted by human CXCL5.

    Neutrophils chemoattracted by human CXCL5.

  • Recombinant Human CXCL10 (IP-10) (carrier-free)
    Human activated T cells chemoattracted

    Human activated T cells chemoattracted by CXCL10.

  • LEGEND MAX™ Human IL-17A/F ELISA Kit with Pre-coated Plates




  • Recombinant Mouse CXCL10 (IP-10) (carrier-free)
    Activated T cells chemoattracted by

    Activated T cells chemoattracted by mouse CXCL10.

  • Recombinant Mouse CXCL1 (KC) (carrier-free)
    Human neutrophils chemoattracted by mouse

    Human neutrophils chemoattracted by mouse CXCL1.

  • Recombinant Human SCF (carrier-free)
    TF-1 cell proliferation induced by

    TF-1 cell proliferation induced by human SCF.

  • Recombinant Mouse G-CSF (carrier-free)
    M-NFS-60 cell proliferation induced by

    M-NFS-60 cell proliferation induced by mouse G-CSF.

  • Recombinant Human CXCL13 (carrier-free)
    Human CXCR5 transfectants chemoattracted by

    Human CXCR5 transfectants chemoattracted by human CXCL13.

  • Recombinant Human M-CSF (carrier-free)
    M-NFS60 cell proliferation induced by

    M-NFS60 cell proliferation induced by human M-CSF.

  • Recombinant Human CXCL11 (ITAC) (carrier-free)
    CXCR3 transfected BaF3 cells chemoattracted

    CXCR3 transfected BaF3 cells chemoattracted by hCXCL11.

  • Recombinant Mouse CXCL11 (ITAC) (carrier-free)
    CXCR3 transfected BaF3 cells chemoattracted

    CXCR3 transfected BaF3 cells chemoattracted by mCXCL11.

  • Recombinant Mouse CCL2 (MCP-1) (carrier-free)
    THP-1 cells chemoattracted by mouse

    THP-1 cells chemoattracted by mouse MCP-1.

  • Recombinant Human G-CSF (carrier-free)
    M-NFS60 cell proliferation induced by

    M-NFS60 cell proliferation induced by human G-CSF.

  • Recombinant Mouse CXCL12 (SDF-1α) (carrier-free)
    Activated human T cells chemoattracted

    Activated human T cells chemoattracted by mouse CXCL12.

  • LEGEND MAX™ Human Soluble DcR3/TNFRSF6B ELISA Kit with Pre-coated Plates




  • Human MCP-1/CCL2 ELISA MAX™ Deluxe




  • Recombinant Mouse CCL17 (TARC) (carrier-free)
    Human CCR4-Baf3 cells attracted by

    Human CCR4-Baf3 cells attracted by mouse CCL17.

  • Recombinant Mouse IL-5 (carrier-free)
    BCL-1 cell proliferation induced by

    BCL-1 cell proliferation induced by mouse IL-5.

  • LEGEND MAX™ Mouse Isthmin ELISA Kit with Pre-coated Plates




  • Recombinant Human CCL8 (MCP-2) (carrier-free)
    THP-1 cells chemoattracted by human

    THP-1 cells chemoattracted by human CCL8 (MCP-2).

  • Recombinant Mouse CCL8 (MCP-2) (carrier-free)
    THP-1 cells chemoattracted by mouse

    THP-1 cells chemoattracted by mouse CCL8 (MCP-2).

  • Brilliant Violet 605™ Streptavidin
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 605™.

  • Brilliant Violet 650™ Streptavidin
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 650™.

  • Recombinant Human IL-33 (carrier-free)
    D10.G4.1 cell proliferation induced by

    D10.G4.1 cell proliferation induced by human IL-33 and co-stimulated with anti-CD3.

  • Recombinant Human IL-7 (carrier-free)
    Proliferation of PHA-activated human PBL

    Proliferation of PHA-activated human PBL induced by human IL-7.

  • LEGEND MAX™ Mouse Latent TGF-β ELISA Kit with Pre-coated Plates




  • Recombinant Human CXCL2 (Groβ) (carrier-free)
    Human neutrophils chemoattracted by human

    Human neutrophils chemoattracted by human CXCL2.

  • Recombinant Human CCL19 (MIP-3β) (carrier-free)
    Baf3-hCCR7 transfectants chemoattracted by human

    Baf3-hCCR7 transfectants chemoattracted by human CCL19.

  • Recombinant Human CCL21 (6CKine) (carrier-free)
    Baf3-hCCR7 transfectants chemoattracted by human

    Baf3-hCCR7 transfectants chemoattracted by human CCL21.

  • LEGEND MAX™ Human Granzyme B ELISA Kit with Pre-coated Plates




  • Recombinant Mouse CCL20 (MIP-3α) (carrier-free)
    Baf3-hCCR6 transfectants chemoattracted by mouse

    Baf3-hCCR6 transfectants chemoattracted by mouse CCL20.

  • Cyto-Last™ Buffer
    PMA+ionomycin-stimulated human peripheral blood lymphocytes

    PMA+ionomycin-stimulated human peripheral blood lymphocytes (for 6 hours in the presence of monensin) were surface stained with CD3 APC and fixed. Cells were then permeabilized and intracellularly stained with human IL-2 (clone MQ1-17H12) PE at day 0 (top), or at day 14 (bottom) after storage in BioLegend's Cyto-Last™ Buffer.





  • Recombinant Human TSLP (carrier-free)
    Baf3-hIL7a/hTSLPR cell proliferation induced by

    Baf3-hIL7a/hTSLPR cell proliferation induced by human TSLP.

  • Recombinant Mouse CXCL2 (MIP-2) (carrier-free)
    Human neutrophils chemoattracted by mouse

    Human neutrophils chemoattracted by mouse CXCL2.

  • LEGEND MAX™ Mouse MCP-1/CCL2 ELISA Kit with Pre-coated Plates




  • LEGEND MAX™ Mouse IFN-β ELISA Kit with Pre-coated Plates




  • CFSE
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) PHA for 5 days. On day 5, cells were harvested and stained with CD4 PerCP. Dot plot (top) was analyzed on live cells. Histogram data (bottom) was analyzed by gating on CD4 positive cells (top).





  • Recombinant Human CCL3 (MIP-1α) (carrier-free)
    CCR5 transfected BaF3 cells chemoattracted

    CCR5 transfected BaF3 cells chemoattracted by human CCL3.

  • LEGEND MAX™ Human APRIL/TNFSF13 ELISA Kit with Pre-coated Plates




  • Recombinant Mouse CCL22 (MDC) (carrier-free)
    Baf3-hCCR4 cells attracted by mouse

    Baf3-hCCR4 cells attracted by mouse CCL22.

  • Recombinant Human CCL1 (I-309) (carrier-free)
    BW5147 cells chemoattracted by human

    BW5147 cells chemoattracted by human CCL1.

  • Rainbow Fluorescent Particles, 1 peak (3.0-3.4 µm) - Mid Range Intensity
    Bead fluorescence at 405 nm

    Bead fluorescence at 405 nm excitation with a 450/50 BP filter (Pacific Blue™ channel).

    Bead fluorescence at 488 nm

    Bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).

    Bead fluorescence at 561 nm

    Bead fluorescence at 561 nm excitation with a 582/15 BP filter (PE channel).

    Rainbow Fluorescent Particles were analysed on a 4-laser BD LSRFortessa™ using detector voltages from a 15-color surface phenotyping assay identifying immune cell subsets in lysed whole blood.

  • LEGEND MAX™ Mouse IL-10 ELISA Kit with Pre-coated Plates




  • DAPI (4',6-Diamidino-2-Phenylindole, Dilactate)
    Human cervical cancer cell line,

    Human cervical cancer cell line, HeLa, was fixed and permeabilized with 100% ethanol, then DNA was stained with 3 µM DAPI.

    Human cervical cancer cell line,

    Human cervical cancer cell line, HeLa, was fixed with 4% paraformaldehyde and stained with 300 nM DAPI.

  • Rainbow Calibration Particles, 6 peaks (3.0-3.4 µm)
    Bead fluorescence at 488 nm

    Bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).

    Bead fluorescence at 640 nm

    Bead fluorescence at 640 nm excitation with a 670/30 BP filter (APC channel) plotted against bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).

    Bead fluorescence at 561 nm

    Bead fluorescence at 561 nm excitation with a 582/15 BP filter (PE channel) plotted against bead fluorescence at 405 nm excitation with a 450/50 BP filter (BV421™ channel).

  • Rainbow Calibration Particles, 8 peaks (3.0-3.4 µm)
    Bead fluorescence at 488 nm

    Bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).

    Bead fluorescence at 640 nm

    Bead fluorescence at 640 nm excitation with a 670/30 BP filter (APC channel) plotted against bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).

    Bead fluorescence at 561 nm

    Bead fluorescence at 561 nm excitation with a 582/15 BP filter (PE channel) plotted against bead fluorescence at 405 nm excitation with a 450/50 BP filter (BV421™ channel).

  • Rainbow Fluorescent Particles, 1 peak (3.0-3.4 µm) - Bright Intensity
    Bead fluorescence at 405 nm

    Bead fluorescence at 405 nm excitation with a 450/50 BP filter (Pacific Blue™ channel).

    Bead fluorescence at 488 nm

    Bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).

    Bead fluorescence at 561 nm

    Bead fluorescence at 561 nm excitation with a 582/15 BP filter (PE channel).

    Rainbow Fluorescent Particles were analysed on a 4-laser BD LSRFortessa™ using detector voltages from a 15-color surface phenotyping assay identifying immune cell subsets in lysed whole blood.

  • Mouse IL-10 ELISA MAX™ Deluxe




  • Mouse IL-10 ELISA MAX™ Standard




  • Recombinant Mouse CCL11 (Eotaxin) (carrier-free)
    CCR3 transfected BaF3 cells chemoattracted

    CCR3 transfected BaF3 cells chemoattracted by mouse CCL11.

  • Recombinant Human CCL11 (Eotaxin) (carrier-free)
    CCR3 transfected BaF3 cells chemoattracted

    CCR3 transfected BaF3 cells chemoattracted by human CCL11.

  • Recombinant Mouse VEGF-164 (carrier-free)
    HUVEC cell proliferation induced by

    HUVEC cell proliferation induced by mouse VEGF-164.

  • Recombinant Human VEGF-121 (carrier-free)
    HUVEC cell proliferation induced by

    HUVEC cell proliferation induced by human VEGF-121.

  • Nuclear Factor Fixation and Permeabilization Buffer Set
    C57BL/6 splenocytes were surface stained

    C57BL/6 splenocytes were surface stained with CD4 PE, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set and intracellularly stained with Helios (clone 22F6) APC.

    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were surface stained with CD4 APC, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set and intracellularly stained with FOXP3 (clone 259D) PE.

  • Recombinant Human TGF-β2 (carrier-free)
    Inhibition of IL-4 induce HT-2

    Inhibition of IL-4 induce HT-2 cell proliferation by TGF-β2.

  • Brilliant Violet 510™ Streptavidin
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram), followed by streptavidin-Brilliant Violet 510™.

  • APC Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was treated (4 hours) with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V APC.

  • PE Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was treated (4 hours) with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V PE.

  • LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit with Pre-coated Plates




  • Recombinant Human CX3CL1 (Fractalkine) (carrier-free)
    BaF3-mCX3CR1 transfectants attracted by human

    BaF3-mCX3CR1 transfectants attracted by human CX3CL1.

  • Recombinant Mouse CX3CL1 (Fractalkine) (carrier-free)
    BaF3-mCX3CR1 transfectants attracted by mouse

    BaF3-mCX3CR1 transfectants attracted by mouse CX3CL1.

  • LEGEND MAX™ Human IL-35 Heterodimer ELISA Kit with Pre-coated Plates




  • Recombinant Human CCL27 (CTACK) (carrier-free)
    BaF3-hCCR10 cells attracted by human

    BaF3-hCCR10 cells attracted by human CCL27.

  • Human IL-34 ELISA MAX™ Deluxe




  • Recombinant Human CCL22 (MDC) (carrier-free)
    BaF3-hCCR4 transfectants attracted by human

    BaF3-hCCR4 transfectants attracted by human CCL22.

  • FITC Annexin V Apoptosis Detection Kit with PI
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with BioLegend's LEAF™ purified anti-CD95 (clone EOS9.1) mAb (Cat. No. 305704) for 4 hours, then stained with FITC Annexin V Apoptosis Detection Kit with PI (Cat. No. 640914)





  • Recombinant Mouse CCL24 (Eotaxin-2) (carrier-free)
    BaF3-mCCR3 cells attracted by mouse

    BaF3-mCCR3 cells attracted by mouse CCL24.

  • Recombinant Human CCL24 (Eotaxin-2) (carrier-free)
    BaF3-mCCR3 cells attracted by human

    BaF3-mCCR3 cells attracted by human CCL24.

  • Recombinant Human VEGF-165 (carrier-free)
    HUVEC cell proliferation induced by

    HUVEC cell proliferation induced by human VEGF-165.

  • LEGEND MAX™ Human IL-34 ELISA Kit with Pre-coated Plates




  • Recombinant Human CCL28 (MEC) (carrier-free)
    BaF3-hCCR10 cells attracted by human

    BaF3-hCCR10 cells attracted by human CCL28.

  • Recombinant Mouse CCL28 (MEC) (carrier-free)
    BaF3-hCCR10 cells attracted by mouse

    BaF3-hCCR10 cells attracted by mouse CCL28.

  • Recombinant Mouse CCL1 (I-309) (carrier-free)
    BW5147 cells attracted by mouse

    BW5147 cells attracted by mouse CCL1.

  • Recombinant Human CCL20 (MIP-3α) (carrier-free)
    BaF3-hCCR6 transfectants attracted by human

    BaF3-hCCR6 transfectants attracted by human CCL20.

  • FITC Annexin V Apoptosis Detection Kit with 7-AAD
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with BioLegend's LEAF™ purified anti-human CD95 (clone EOS9.1) mAb (Cat. No. 305704) for 4 hours, then stained with FITC Annexin V Apoptosis Detection Kit with 7-AAD (Cat. No. 640922).





  • Recombinant Human CCL26 (Eotaxin-3) (carrier-free)
    BaF3-mCCR3 cells attracted by human

    BaF3-mCCR3 cells attracted by human CCL26.

  • Recombinant Human CXCL17 (VCC-1) (carrier-free)
    Bactericidal activity of human CXCL17

    Bactericidal activity of human CXCL17 against E.coli.

  • Recombinant Mouse CXCL17 (VCC-1) (carrier-free)
    Bactericidal activity of mouse CXCL17

    Bactericidal activity of mouse CXCL17 against E.coli.

  • Recombinant Human EGF (carrier-free)
    Inhibition of A431 cell proliferation

    Inhibition of A431 cell proliferation by human EGF.

  • Recombinant Mouse EGF (carrier-free)
    Inhibition of A431 cell proliferation

    Inhibition of A431 cell proliferation by mouse EGF.

  • LEGENDScreen™ Human Cell Screening (PE) Kit




  • Recombinant Human CCL7 (MCP-3) (carrier-free)
    THP-1 cells chemoattracted by human

    THP-1 cells chemoattracted by human CCL7 (MCP-3).

  • Recombinant Human TGF-β3 (carrier-free)
    TGF-β3 effect on HT-2 cell

    TGF-β3 effect on HT-2 cell proliferation induced by mouse IL-4.

  • Recombinant Human IL-11 (carrier-free)
    7TD1 cell proliferation induced by

    7TD1 cell proliferation induced by human IL-11.

  • Recombinant Mouse IL-11 (carrier-free)
    7TD1 cell proliferation induced by

    7TD1 cell proliferation induced by mouse IL-11.

  • Recombinant Mouse CCL7 (MCP-3) (carrier-free)
    THP-1 cells chemoattracted by mouse

    THP-1 cells chemoattracted by mouse CCL7 (MCP-3).

  • Recombinant Mouse CCL9 (MIP-1γ) (carrier-free)
    THP-1 cells attracted by mouse

    THP-1 cells attracted by mouse CCL9.

  • Recombinant Human CXCL3 (GRO-γ) (carrier-free)
    Neutrophils attracted by human CXCL3.

    Neutrophils attracted by human CXCL3.

  • Recombinant Mouse CCL21 (6CKine) (carrier-free)
    Baf3-hCCR7 cells chemoattracted by mouse

    Baf3-hCCR7 cells chemoattracted by mouse CCL21.

  • LEGEND MAX™ Human Myeloperoxidase ELISA Kit with Pre-coated Plates




  • Recombinant Human CXCL6 (GCP2) (carrier-free)
    Neutrophils attracted by human CXCL6.

    Neutrophils attracted by human CXCL6.

  • Recombinant Human IL-21 (carrier-free)
    Human IL-21 induced-production of IFNγ

    Human IL-21 induced-production of IFNγ by NK-92 cells.

  • Recombinant Mouse CXCL13 (carrier-free)
    BaF3-mCXCR5 cells attracted by mouse

    BaF3-mCXCR5 cells attracted by mouse CXCL13.

  • LEGEND MAX™ Human sVCAM-1/CD106 ELISA Kit with Pre-coated Plates




  • Recombinant Human CXCL7 (NAP-2) (carrier-free)
    Neutrophils chemoattracted by human CXCL7.

    Neutrophils chemoattracted by human CXCL7.

  • Recombinant Mouse CXCL7 (NAP-2) (carrier-free)
    Human neutrophils attracted by mouse

    Human neutrophils attracted by mouse CXCL7.

  • LEGEND MAX™ Human sICAM-1/CD54 ELISA Kit with Pre-coated Plates




  • Recombinant Mouse CCL25 (TECK) (carrier-free)
    Baf3-hCCR9 cells chemoattracted by mouse

    Baf3-hCCR9 cells chemoattracted by mouse CCL25.

  • Recombinant Human CCL25 (TECK) (carrier-free)
    BaF3-hCCR9 cells attracted by human

    BaF3-hCCR9 cells attracted by human CCL25.

  • Recombinant Human CCL23 (MPIF-1) (Arg22-Ans120) (carrier-free)
    THP-1 cells attracted by human

    THP-1 cells attracted by human CCL23.

  • Recombinant Human CCL23 (MPIF-1) (Arg46-Ans120) (carrier-free)
    THP-1 cells attracted by human

    THP-1 cells attracted by human CCL23-short.

  • Zombie Aqua™ Fixable Viability Kit
    One day old splenocytes were

    One day old splenocytes were stained with Zombie Aqua™ and analyzed without fixation (blue) or analyzed after fixation and permeabilization (red). Cells alone without Zombie Aqua™ staining are indicated in black.

    One day old unfixed murine

    One day old unfixed murine splenocytes, with a 1:500 dilution of Zombie Aqua™, were incubated for 15 minutes, followed by the addition of a seven-antibody cocktail without wash (red) using the No-wash Sequential Staining protocol. Cells were then fixed for 20 minutes in FOXP3 fix/perm buffer prior to analysis (blue). Live, unstained cells are indicated in black.

  • LEGEND MAX™ Human MMP-9 ELISA Kit with Pre-coated Plates




  • Recombinant Human Erythropoietin (EPO) (carrier-free)
    TF-1 cell proliferation induced by

    TF-1 cell proliferation induced by human EPO.

  • Recombinant Human CCL14 (HCC-1) (carrier-free)
    THP-1 cells attracted by human

    THP-1 cells attracted by human CCL14.

  • Recombinant Mouse IL-25 (IL-17E) (insect expressed, carrier-free)
    Mouse IL-25 binding to recombinant

    Mouse IL-25 binding to recombinant IL-17BR/Fc chimera detected by functional ELISA.

  • LEGEND MAX™ Human CXCL10 (IP-10) ELISA Kit with Pre-coated Plates




  • Recombinant Human CCL15 (MIP-1δ) (carrier-free)
    THP-1 cells attracted by human

    THP-1 cells attracted by human CCL15.

  • Recombinant Human FASL (TNFSF6) (carrier-free)
    Apoptotic cell death induced by

    Apoptotic cell death induced by human FASL in Jurkat cells.

  • Recombinant Mouse CXCL14 (BRAK) (carrier-free)
    THP-1 cells attracted by mouse

    THP-1 cells attracted by mouse CXCL14.

  • Recombinant Human CXCL14 (BRAK) (carrier-free)
    THP-1 cells attracted by human

    THP-1 cells attracted by human CXCL14.

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