The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
For immunofluorescent staining, the suggested use of this reagent is 20 µl per 106 cells or 20 µl per 100 µl of whole blood.
Application Notes:
This RTK4530 immunoglobulin product is useful as an isotype-matched control for immunofluorescent staining (surface) for flow cytometric analysis.
Application References:
1. Cervantes-Barragan, L., et al., 2007. Blood 109:1131. 2. Zeiser, R., et al., 2007. Blood 109:2225.
Description:
The isotype of RTK4530 immunoglobulin is rat IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
