The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
For immunofluorescent staining, the suggested use of this reagent is 20 µl per 106 cells or 20 µl per 100 µl of whole blood.
Application Notes:
This RTK4530 immunoglobulin product is useful as an isotype-matched control for immunofluorescent staining (surface) for flow cytometric analysis.
Application References:
1. Cervantes-Barragan, L., et al., 2007. Blood 109:1131. 2. Zeiser, R., et al., 2007. Blood 109:2225.
Description:
The isotype of RTK4530 immunoglobulin is rat IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
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