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PE Mouse IgG2a, κ Isotype Ctrl (FC) Antibody
PE Mouse IgG2a, κ Isotype Ctrl (FC) Antibody
400213 25 tests $50.00     
400214 100 tests $110.00     
Product Details
Clone: MOPC-173
Isotype: Mouse IgG2a, κ
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody, and is formulated for cell surface immunofluorescent staining as negative control.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Enter Lot#:   
Application
Notes:

The MOPC-173 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofleorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400224) as negative control.
Application
References:

1. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
2. Burman AC, et al. 2007. Blood 110:1064.
3. Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.
4. Podolin PL, et al. 2008. J. Immunol.. 180:7989. PubMed



Description:

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is Mouse IgG2a,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
GeneID: NA
Latest Publications: View the latest IgG2a, kappa Isotype Ctrl articles on HighwirePress.com
UniProt: View information about IgG2a, kappa Isotype Ctrl on UniProt.org
Keywords: PE Mouse IgG2a, κ Isotype Ctrl (FC), MOPC-173, PE, Immunology, Antibodies
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