The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality Tested ELISA*, WB* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this fluorochrome conjugated antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
This PE conjugated polyclonal goat anti-rat IgG antibody is useful for immunofluorescent staining and flow cytometric analysis.
Description:
This polyclonal goat anti-rat IgG antibody reacts with the heavy chains of rat IgG and with the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with mouse, human, bovine, horse, and rabbit immunoglobulins, but it may cross-react with other subclasses of rat immunoglobulins.
