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PE/Cy7 Streptavidin
PE/Cy7 Streptavidin
405206 100 µg ¥35,000     
Product Details
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated Streptavidin.
Concentration: 0.2 mg/ml
Storage & Handling: The Streptavidin-PE/Cy7 solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested
Recommended Usage:

Each lot of this Streptavidin-PE/Cy7 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
Notes:

Streptavidin - Phycoerythrin/Cy7 (PE/Cy7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
  Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application
References:

1. Kamimura D, et al. 2006. J. Immunol. 177:306.
2. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
3. Anderson SM, et al. 2007. J. Exp. Med. doi:10.1084/jem.20062571.PubMed
4. Chappaz S, et al. 2010. J. Immunol. 184:3562. PubMed
5. Weber SE, et al. 2011. J. Immunol. 186:432. PubMed
6. Chamoto, K., et al. 2011. Am J Respir Cel Mol Biol. PubMed.
7. Ahuja, A., et al. 2011. J. Immunol. 187:3888. PubMed
8. Chamoto K, et al. 2012. Am J Respir Cell Mol Biol. 46:283. PubMed.


See PE/Cy7 spectral data



Description:

Streptavidin binds to biotin with high affinity. Streptavidin - Phycoerythrin/Cy7 (PE/Cy7) is useful for detecting biotinylated antibodies. The excitation of PE/Cy7 by 488 nm laser light induces a light emission maximum of 775 nm.
Other Names: Streptavidin-Phycoerythrin/Cy7, SAv-PE/Cy7
GeneID: NA
Latest Publications: View the latest Streptavidin articles on HighwirePress.com
UniProt: View information about Streptavidin on UniProt.org
Keywords: PE/Cy7 Streptavidin, PE/Cy7, Streptavidin-Phycoerythrin/Cy7, SAv-PE/Cy7, Mouse, Human, Rat, Immunology, Antibodies
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