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The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration:
test sizes: lot-specific; µg size: 0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. For test sizes, please note that they are transitioning from 20 ul to 5 ul per test. Check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. Read more at www.biolegend.com/testsize regarding the test size change. For µg sizes, use at concentrations comparable to those of the specific antibody of interest.
COA:
Application Notes:
The MOPC-173 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofleorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400224) as negative control.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Luckashenak NA, et al. 2006. J. Immunol. 177:5177. 2. Burman AC, et al. 2007. Blood 110:1064. 3. Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200. 4. Podolin PL, et al. 2008. J. Immunol.. 180:7989. PubMed
Description:
The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is Mouse IgG2a,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
