The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
ICFC7,8,12,13 - Quality Tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as capture antibody and recombinant mouse IFN-γ (Cat. No. 564601) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjuncrion with biotinylated R4-6A2 antibody (Cat. No. 505704) as detecting antibody and recombinant mouse IFN-γ (Cat. No. 564601) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Flow Cytometry7,8,12,13,20: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization1-3,9,10: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.
Application References:
1. Abrams, J., et al., 1992. Immunol. Rev. 127: 5. 2. Sander, B., et al., 1993. J. Immunol. Meth. 166: 201. 3. Abrams, J., et al., 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20 4. Yang, X., et al., 1993. J. Immunoassay 14: 129. 5. Klinman, D., et al., 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. 6. Sander, B., et al., 1991. Immunol. Rev. 119: 65. 7. Ferrick, D., et al., 1995. Nature 373: 255. 8. Ko, S-Y., et al., 2005. J. Immunol. 175:3309.PubMed 9. Peterson, K. E., et al., 2000. J. Virol. 74:5363. 10. DeKrey, G. K., et al., 1998. Infect. Immun. 66:827. 11. Dzhagalov, I., et al 2007. J. Immunol. 178:2113. 12. Lawson, B. R., et al., 2007. J. Immunol. 178:5366. 13. Lee, J-W., et al., 2006. Nature Immunol. 8:181.PubMed 14. Xu, G., et al 2007. J. Immunol. 179:5358.PubMed 15. Montfort, M. et al.2004 J. Immunol 173:4084.J.Immunol 16. Haring, JS., et al. 2008. J immunol. 180:2855. PubMed 17. Jordan, JM.,et al. 2008. Infect Immune. 76:3717. PubMed 18. Tonkin, DR., et al. , 2008. J Immunol. 181:4516. PubMed 19. Charles, N., et al., 2010. Nat. Med. 16:701. (FC) PubMed 20. Cui, Y., et al., 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
PMA/Ionomycin-stimulated (6hrs) C57BL/6 mouse splenocytes stained with B220 (RA3-6B2) APC and XMG1.2 PE/Cy7
Description:
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The XMG1.2 antibody reacts with mouse interferon-γ (IFN-γ). The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Other Names:
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Structure:
Cytokine; dimer; 40-80 kD (Mammalian)
Regulation:
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Cellular Sources:
CD8+ and CD4+ T cells, NK cells
Cellular Targets:
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors:
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity/Activities:
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Antigen References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. De Maeyer, E., et al., 1992. Curr. Opin. Immunol. 4:321. 3. Farrar, M., et al., 1993. Annu. Rev. Immunol. 11:571. 4. Gray, P., et al., 1987. Lymphokines 13:151.
