The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections, and induction of B cell proliferation1.
Application References:
1. Oliver, A.M., et al., 1997. J. Immunol. 158:1108. 2. Howard, M., et al., 1993. Science 262:1056.
C57BL/6 mouse splenocytes stained with 90 PE/Cy7
Description:
CD38 is a 42 kD glycoprotein, also known as T10. It is an ADP-ribosyl hydrolase, expressed on B cells, NK cells, subset of T cells, brain, muscle, and kidney. In the mouse, CD38 expression is downregulated on mouse germinal center B cells and plasma cells, whereas this is not the case for humans. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, as well as adhesion and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
Other Names:
T10
Structure:
ADP-ribosyl hydrolase, 42 kD
Distribution:
B cells, NK cells, T cell subset, brain, muscle, kidney
Function:
Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand Receptor:
CD31, hyaluronic acid
Antigen References:
1. Barclay, A.N. et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Shubinsky, G., et al., 1997. Immunity 7:315. 3. Cesano, A., et al., 1998. J. Immunol. 160:1106. 4. Cockayne, D.A., et al., 1998. Blood 92:1324.
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