The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Farr A, et al. 1991. J Histochem Cytochem 39:645 (Fc, IHC) 2. Dooley J et al. 2005. J Immunol 175:4331 (FC, IHC)
EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion.It may also function as a growth factor receptor.It is though to be involved in maintaining cells in position during proliferation.Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324).EpCAM is considered important in tumor biology.
Other Names:
CD326, EGP40, MIC18, TROP1, KSA
Structure:
40 kd Single-pass type 1 glycoprotein with 293 amino acids, which consists of a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain and a 26 aa cytoplasmic domain. The extracellular domain contains two repeats of epiderma
Distribution:
Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
