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The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Maier H, et al. 2007. J. Immunol. 178:2714. 2. Meng Q, et al. 2006. Invest. Ophth. Vis. Sci. 47:444. PubMed
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) PE/Cy7 (orange line) or rat IgG2b, κ PE/Cy7 (purple line).
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T lymphocyte proliferation and production of IL-10 and IFN-g, in an IL-2-dependent and a PDCD1-independent manner. Its interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Other Names:
B7-H1, PD-L1
Structure:
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution:
T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
Ligand Receptor:
PD-1 (PDCD1)
Antigen References:
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239. 2. Dong H, et al. 1999. Nat. Med. 5:1365. 3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with purified anti-CD274 (clone 10F.9G2) (green line) or purified rat IgG2b, κ PE/Cy7 (pink line).
LEAF™ Purified anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with LEAF™ purified anti-CD274 (clone 10F.9G2) (green line) or purified rat IgG2b, κ (red line) followed by Sav-PE.
Biotin anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with biotinylated anti-CD274 (clone 10F.9G2) (green line) or biotinylated rat IgG2b, κ (pink line) followed by Sav-PE.
PE anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) PE (filled histogram) or rat IgG2b, κ PE (open histogram).
C57BL/6 mouse splenocytes were stained with CD274 (clone 10F.9G2) Brilliant Violet 421™ (filled histogram), or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
APC anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) APC (pink line) or rat IgG2b, κ APC (purple line).
PE/Cy7 anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) PE/Cy7 (orange line) or rat IgG2b, κ PE/Cy7 (purple line).
