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The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Saito K, et al. 2008. J. Immunol. 181:6889. PubMed 2. Ueha S, et al. 2007. J. Leukoc. Biol. 82:1230. PubMed 3. Sharma R, et al. 2009 J. Immunol. 183:1065 (FC) PubMed 4. Dogan R, et al. 2011. J. Leukoc. Biol. 89:93. PubMed
Multiple-immunized Balb/c lymph node cells stained with 2G12 PE/Cy7 and CD3e (145-2C11) APC
Multiple-immunized Balb/c lymph node cells stained with Armenian Hamster IgG PE/Cy7 isotype control and CD3e (145-2C11) APC
Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85% identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).
Memory T cells, macrophages, platelets, basophils, Th2 cells, and Tregs
Function:
CCR4 and its ligands (CCL17 and CCL22) are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
Ligand Receptor:
CCl17 and CCL22
Interaction:
Interaction between CCR4 and its ligand TARC/CCL17 on activated endothelial cells mediates T cell extravasation by stimulating integrin-dependent adhesion
Antigen References:
1. Biederman T, et al. 2002. Eur. J. Immun. 32:3171. 2. Mirshahpanah P, et al. 2008. Exp. Dermatol. 17:30. 3. Kusumoto M, et al. 2007. J. Interferon Cytokine Res 27:901. 4. Clark RA and Kupper TS. 2006. Blood 109:194. 5. Lee I, et al. 2005. J. Exp. Med. 201:1037.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse CD194 (CCR4)
Hyper-immunized BALB/c splenocytes stained with GK1.5 (CD4) APC and 2G12 PE
APC anti-mouse CD194 (CCR4)
Hyper-immunized BALB/c splenocytes stained with 145-2C11 (CD3) PE and 2G12 APC
PE/Cy7 anti-mouse CD194 (CCR4)
Multiple-immunized Balb/c lymph node cells stained with 2G12 PE/Cy7 and CD3e (145-2C11) APC
Multiple-immunized Balb/c lymph node cells stained with Armenian Hamster IgG PE/Cy7 isotype control and CD3e (145-2C11) APC
Biotin anti-mouse CD194 (CCR4)
Hyper-immunized Balb/c splenocytes stained with CD3 APC and biotinylated 2G12, followed by Sav-PE
Brilliant Violet 421™ anti-mouse CD194 (CCR4)
Splenocytes from a 12 month old BALB/c mouse were stained with CD4 FITC and CCR4 (clone 2G12) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
