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The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Chen K, et al. 2009. Nat. Immunol. 10:889. 2. Lee CH, et al. 2005. J. Exp. Med. 203:63. 3. Sutter JA, et al. 2008. Clin. Immunol. 126:282.
IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.
Other Names:
Ig delta chain C region
Structure:
Exists in a transmembranal and a soluble form
Distribution:
Naïve B cells
Function:
Antigen binding, B cell activation
Interaction:
The CD79a/CD79b heterodimer
Antigen References:
1. Geisberger R, et al. 2006. Immunology 118:429. 2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789. 3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
