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The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Nishimura M, et al. 2002. J. Immunol. 168:6173. 2. Nanki T, et al. 2002. Arthritis Rheum. 46:2878. 3. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837.
Human peripheral blood lymphocytes were stained with CD3 FITC and CX3CR1 (clone 2A9-1) PE/Cy7 (top) or rat IgG2b, κ PE/Cy7 isotype control (bottom).
CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.
Other Names:
V28, GPR13, Chemokine C-X3-C receptor 1
Structure:
G-protein-coupled seven transmembrane receptor
Distribution:
NK cells, T cell subset, monocytes/macrophages, dendritic cells
Function:
cell adhesion and migration
Ligand Receptor:
CX3CL1 (Fractalkine, FKN, neurotactin)
Antigen References:
1. Imai T, et al. 1997. Cell. 91:521. 2. Fong AM, et al. 1998. J. Exp. Med. 188:1413. 3. Auffray C, et al. 2009. J. Exp. Med. 206:595.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE/Cy7 anti-human CX3CR1
Human peripheral blood lymphocytes were stained with CD3 FITC and CX3CR1 (clone 2A9-1) PE/Cy7 (top) or rat IgG2b, κ PE/Cy7 isotype control (bottom).
Purified anti-human CX3CR1
Human peripheral blood lymphocytes stained with CD3 APC (UCHT1) and purified 2A9-1 conjugated with PE
PE anti-human CX3CR1
Human peripheral blood lymphocytes stained with CD3 APC (UCHT1) and 2A9-1 PE
FITC anti-human CX3CR1
Human peripheral blood lymphocytes stained with 2A9-1 FITC and anti-CD3 (UCHT1) APC
Alexa Fluor® 647 anti-human CX3CR1
Human peripheral blood lymphocytes stained with 2A9-1 Alexa Fluor® 647 and CD3 (OKT3) PE
APC anti-human CX3CR1
Human peripheral blood lymphocytes stained with CD3 FITC and 2A9-1 APC (top) or rat IgG2b, κ APC isotype control(bottom)
