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The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation2,3. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 304310).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Jeong S-H, et al. 2004. J. Virol. 78:6995. (Costim) 3. Vogel TU, et al. 2002. J. Immunol. 169:4511. (Costim) 4. Kleinewietfeld M, et al. 2009. Blood 113:827. (FC) PubMed 5. Palacious F, et al. 2010. Blood 115:4488. PubMed 6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. 8. Mattapallil MJ, et al. 2011 J. Immunol. 187:197. PubMed.
Human peripheral blood lymphocytes stained with 9F10 PE/Cy7
CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells. The 9F10 antibody is able to costimulate T cell activation.
Other Names:
VLA-4 α chain, α4 integrin, Integrin α4 chain
Structure:
Integrin, type I transmembrane glycoprotein, 150 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 APC
LEAF™ Purified anti-human CD49d
Human peripheral blood lymphocytes stained with LEAF™ purified 9F10, followed by anti-mouse IgGs FITC
PE anti-human CD49d
Human peripheral blood lymphocytes stained
with 9F10 PE
PE/Cy5 anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 PE/CY5
Purified anti-human CD49d
Human peripheral blood lymphocytes stained with purified 9F10, followed by anti-mouse IgGs FITC
PerCP/Cy5.5 anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 PerCP/Cy5.5
PE/Cy7 anti-human CD49d
Human peripheral blood lymphocytes stained with 9F10 PE/Cy7
