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The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Toldi, G., et al. 2011. Am. J. Reprod. Immunol. doi:10.1111/j.1600-0897. (FC) PubMed
Human peripheral blood lymphocytes stained with CD3 (UCHT1) FITC and TG6/CCR4 PE/Cy7
CCR4 is a CC chemokine receptor, and it was clone from a human basophylic cell line KU-812 in 1995. (1). CCR4 binds CCL17 and CCL22 and is expressed on stimulated and unstimulated T, and B cells, basophils, monocytes, and NK cells. Human Th2 cells are characterized by the expression of CCR4 and CCR8 (2), and these receptors are regulated differently during Th2 development (3). Human peripheral blood Tregs can be divided into two distinct populations based on the expression of CCR4. Freshly isolated Tregs express CCR4 and presumably represent memory-type Tregs, and CCR4(-) Tregs require anti-CD3 Ab-mediated activation to acquire a regulatory activity. Depletion of CCR4(+) T cells leads to Th1-type polarization of CD4(+) T cells and augmentation of CD8(+) T cell responses to tumor Ags (4). CCR4 and its ligands are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases (5).
CCR4 is expressed in stimulated and unstimulated T, and B cells, basophils, monocytes, NK, Th2 and Treg cells
Function:
Migration of inflammatory and regulatory cells to the target tissues
Ligand Receptor:
CCL17 and CCL22
Antigen References:
1) Power et al. 1995 J. Biol. Chem., 270:19495. 2) Katschke KJ, et al. 2001, Arthritis Rheum, 44:1022. 3) Colantonio L, et al. 2002, Eur. J. Immunol., 32:1264. 4) Jakubzick, C. et al. 2004 Am J Pathol 165:1211 5) Morimoto Y, et al. 2005, J Leukoc Bio, 78:753 6) Baatar J, et al. 2007, J. Immunol., 178(8):4891. 7) Kusumoto M, et al. 2007 J Interf Cytok Res 27:901.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE/Cy7 anti-human CD194 (CCR4)
Human peripheral blood lymphocytes stained with CD3 (UCHT1) FITC and TG6/CCR4 PE/Cy7
Alexa Fluor® 647 anti-human CD194 (CCR4)
Human peripheral blood lymphocytes stained with CD3 (UCHT1) PE and TG6/CCR4 Alexa Fluor® 647
PerCP/Cy5.5 anti-human CD194 (CCR4)
Human peripheral blood lymphocytes stained with CD4 (RPA-T4) PE and TG6/CCR4 PerCP/Cy5.5
