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The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Excitation Laser:
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and immunofluorescence microscopy.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.
Other Names:
LPS receptor
Structure:
GPI-linked membrane glycoprotein, 53-55 kD
Distribution:
Monocytes, macrophages, granulocytes (low)
Function:
LPS receptor, clearance of Gram-negative pathogens
Ligand Receptor:
LPS
Antigen References:
1. Stocks S, et al. 1990. Biochem. J. 268:275. 2. Wright S, et al. 1990. Science 249:1434.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD14
Human peripheral blood monocytes stained with purified HCD14, followed by anti-mouse IgG FITC
FITC anti-human CD14
Human peripheral blood monocytes stained with HCD14 FITC
PE anti-human CD14
Human peripheral blood monocytes stained with HCD14 PE
APC anti-human CD14
Human peripheral blood monocytes stained with HCD14 APC
Alexa Fluor® 488 anti-human CD14
Human peripheral blood monocytes stained with HCD14 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD14
Human peripheral blood monocytes stained with HCD14 alexa Fluor® 647
Alexa Fluor® 700 anti-human CD14
Human peripheral blood monocytes stained with HCD14 Alexa Flour® 700
Pacific Blue™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (HCD14) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
PE/Cy7 anti-human CD14
Human peripheral blood monocytes stained with HCD14 PE/Cy7
APC/Cy7 anti-human CD14
Human peripheral blood monocytes stained with HCD14 APC/Cy7
PerCP/Cy5.5 anti-human CD14
Human peripheral blood monocytes stained with HCD14 PerCP/Cy5.5
Biotin anti-human CD14
Human peripheral blood monocytes stained with biotinylated HCD14 and then detected with Sav-PE
