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The CD11c antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Storage & Handling:
The CD11c antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Clone 3.9 preferentially binds the activated form of CD11c, is specific for I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent.12 Additional reported applications (for the relevant formats) include: immunohistochemical staining4 of acetone-fixed frozen tissue sections, and functional assays5,6. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 301616).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. 3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. 4. Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC) 5. Ottonello L, et al. 1999. Blood 93:3505. 6. Metelitsa LS, et al. 2002. Blood 99:4166. 7. Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed 8. Ihanus E, et al. 2007. Blood 109:802-810. 9. Gurer C, et al. 2008. Blood 112:1231.PubMed 10. Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed 11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 12. Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)
Human peripheral blood granulocytes stained with 3.9 PE/Cy7
CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
Other Names:
Integrin αX subunit, CR4, p150
Structure:
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Ligand Receptor:
CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Antigen References:
1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE
PE anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE
Purified anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE
LEAF™ Purified anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE
PE/Cy7 anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE/Cy7
PE/Cy5 anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PE/Cy5
Biotin anti-human CD11c
Human peripheral blood granulocytes stained with biotinylated 3.9, followed by Sav-PE
APC anti-human CD11c
Human peripheral blood monocytes stained with 3.9 APC
Alexa Fluor® 488 anti-human CD11c
Human peripheral blood monocytes stained with 3.9 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD11c
Human peripheral blood monocytes stained with 3.9 Alexa Fluor® 647
Pacific Blue™ anti-human CD11c
Human peripheral blood monocytes stained with anti-human CD11c (clone 3.9) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram)
PerCP/Cy5.5 anti-human CD11c
Human peripheral blood granulocytes stained with 3.9 PerCP/Cy5.5
Brilliant Violet 421™ anti-human CD11c
Human peripheral blood granulocytes were stained with CD11c (clone 3.9) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
