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The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The H57-597 antibody does not cross-react with γ/δ TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate α/β TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 109214).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296. 2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP) 3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim) 4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) 5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete) 6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete) 7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC) 8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC) 9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC) 10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed 11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed 12. Rashe SS, et al. 2011. Diabetes. 60:1716. PubMed.
C57BL/6 mouse splenocytes stained with H57-597 PE/Cy5.
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.
Other Names:
TCR-β chain, TCR-β, β-TCR
Structure:
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution:
Majority of T cells and thymocytes (correlated to differentiation)
Function:
Antigen recognition, T cell activation
Ligand Receptor:
Peptide bound-MHC class I and II
Antigen References:
1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523. 2. Huppa JB, et al. 2003. Nat. Immunol. 4:749. 3. Kubo R, et al. 1989. J. Immunol. 142:2736.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 APC
Biotin anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with biotinylated H57-597, followed by Sav-PE
FITC anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 FITC
LEAF™ Purified anti-mouse TCR β chain
C57BL/6 CD3+ splenocytes stained
with H57-597 PE and TCRγ/δ (UC7-
13D5) FITC
PE anti-mouse TCR β chain
C57BL/6 CD3+ splenocytes stained
with H57-597 PE and TCRγ/δ (UC7-
13D5) FITC
PE/Cy5 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 PE/Cy5.
Purified anti-mouse TCR β chain
C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC
Alexa Fluor® 488 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 Alexa Fluor® 647
PE/Cy7 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 PE/Cy7
Alexa Fluor® 700 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® 700
Pacific Blue™ anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 Pacific Blue™
Brilliant Violet 421™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 421™ (filled histrogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram).
PerCP/Cy5.5 anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 PerCP/Cy5.5
Brilliant Violet 570™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 570™ (filled histogram) or Armenian hamster IgG Brilliant Violet 570™ isotype control (open histogram).
