The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections.
Application References:
1. Dong, C., et al., 2001. Nature 409(6816):97. 2. Walker, L.S.K., et al., 2003. J. Immunol. 170:91. 3. Wigren, M., et al. 2009. Circ Res. 104:e62. PubMed This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
ConA-stimulated C57BL/6 mouse splenocytes stained with 15F9 PE/Cy5
Description:
CD278 is a 47-57 kD protein, also known as inducible costimulatory molecule (ICOS), or H4. This protein is homologous to the CD28/CTLA-4 proteins. It forms homodimers and is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. It has also been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.
Other Names:
Inducible COStimulatory molecule, H4, CD278
Structure:
CD28/CTLA-4, 47-57kD
Distribution:
Activated T cells, subset of thymocytes
Function:
Costimulates T cell activation, proliferation, humoral immune response
Ligand Receptor:
B7h/B7RP-1/GL-50
Antigen References:
1. Rudd, C.E., et al., 2003. Nat. Rev. Immunol. 3:544. 2. McAdam, A. J., et al., 2000. J. Immunol. 165:5035. 3. Mak, T.W., et al., 2003. Nat. Immunol. 4:765
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