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The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser:
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Application Notes:
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Other Names:
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Structure:
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution:
All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function:
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand Receptor:
Hyaluronan, MIP-1β, fibronectin, collagen
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Haynes BF, et al. 1991. Cancer Cells 3:347. 3. Goldstein LA, et al. 1989. Cell 56:1063. 4. Mikecz K, et al. 1995. Nat. Med. 1:558. 5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134. 6. Liu T, et al. 2009. Biol. Direct 4:40.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with IM7 APC
Biotin anti-mouse/human CD44
C57BL/6 mouse splenocytes were stained with biotinylated CD44 (clone IM7) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE.
FITC anti-mouse/human CD44
C57BL/6 splenocytes stained with IM7 FITC
Human peripheral blood lymphocytes stained with IM7 FITC
LEAF™ Purified anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with LEAF™ purified IM7, followed by anti-rat IgG FITC
PE/Cy5 anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with IM7 PE/Cy5
Purified anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with purified IM7, followed by anti-rat IgG FITC
PE anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with IM7 PE
Human peripheral blood lymphocytes stained with IM7 PE
Alexa Fluor® 488 anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with IM7 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with IM7 Alexa Fluor® 647
Pacific Blue™ anti-mouse/human CD44
C57BL/6 Mouse Splenocytes stained with IM7 Pacific Blue™
Alexa Fluor® 700 anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with IM7 Alexa Fluor® 700
PE/Cy7 anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with IM7 PE/Cy7
APC/Cy7 anti-mouse/human CD44
C57BL/6 splenocytes stained with IM7 APC/Cy7
PerCP/Cy5.5 anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with IM7 PerCP/Cy5.5
PerCP anti-mouse/human CD44
C57BL/6 splenocytes stained with IM7 PerCP
Brilliant Violet 421™ anti-mouse/human CD44
C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-mouse/human CD44
C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 570™ (filled histogram) or rat IgG2b, κ Brilliant Violet 570™ isotype control (open histogram).
Brilliant Violet 785™ anti-mouse/human CD44
C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 785™.
Brilliant Violet 510™ anti-mouse/human CD44
C57BL/6 mouse splenocytes were stained with CD44 (clone IM7) Brilliant Violet 510™.
Ultra-LEAF™ Purified anti-mouse/human CD44
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD44 (clone IM7), followed by anti-rat IgG FITC.
