he antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103408).
Application References:
1. Kato, K., et al., 1992. J. Exp. Med. 176:1241. 2. Qin, L., et al., 1994. J. Exp. Med. 179:341.
C57BL/6 splenocytes stained with HM48-1 PE/CY5
Description:
CD48 is a 45 kD GPI-anchored glycoprotein, also known as BCM1, Blast-1 (human), or OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells, and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244. The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions.
Other Names:
BCM1, Blast-1 (human), OX-45 (rat)
Structure:
Ig superfamily, 45 kD
Distribution:
T and B cells, monocytes/macrophages
Function:
Adhesion, T cell recognition
Ligand Receptor:
CD2, CD244
Antigen References:
1. Barclay, A.N., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Flament, C., et al., 1996. Hum. Immunol. 46:82. 3. Van der Merwe, P.A., et al., 1995. Curr. Biol. 5:74. 4. Latchman, Y., et al., 1998. J. Immunol. 161:5809.
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