he antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103408).
Application References:
1. Kato, K., et al., 1992. J. Exp. Med. 176:1241. 2. Qin, L., et al., 1994. J. Exp. Med. 179:341.
C57BL/6 splenocytes stained with HM48-1 PE/CY5
Description:
CD48 is a 45 kD GPI-anchored glycoprotein, also known as BCM1, Blast-1 (human), or OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells, and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244. The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions.
Other Names:
BCM1, Blast-1 (human), OX-45 (rat)
Structure:
Ig superfamily, 45 kD
Distribution:
T and B cells, monocytes/macrophages
Function:
Adhesion, T cell recognition
Ligand Receptor:
CD2, CD244
Antigen References:
1. Barclay, A.N., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Flament, C., et al., 1996. Hum. Immunol. 46:82. 3. Van der Merwe, P.A., et al., 1995. Curr. Biol. 5:74. 4. Latchman, Y., et al., 1998. J. Immunol. 161:5809.
