The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 102112).
Application References:
1. Gross, J.A., et al., 1992. J. Immunol. 149:380. 2. Cibotti, R., et al., 1997. Immunity 6:245. 3. Masten, B.J., et al., 1997. Am. J. Respir. Cell Mol. Biol. 16:335. 4. Nishio, M., et al., 1996. J. Immunol. 157:4347. 5. Zhang, N. and He, Y-W., 2005. J. Exp. Med. 202:395. 6. Terrazas, L. I., et al., 2005. Intl. J. Parasitology. 35:1349. 7. Perchonock, C. E., et al., 2006. Mol Cell Biol. 26(16):6005. 8. Wang, W., et al., 2007. J. Immunol. 178:4885. 9. Pua, H. H., et al., 2007. J. Exp. Med. 204:25. 10. Perchonock, C. E., et al., 2007. J. Immunol. 179:1768. 11. Barbi, J., et al., 2007. Blood 110:2215. This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
C57BL/6 splenocytes stained with
37.51 PE and CD3 FITC
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and 37.51 PE/Cy5
Description:
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
Other Names:
Tp44, T44
Structure:
Ig superfamily, 44 kD
Distribution:
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
Function:
Costimulates T and NK cells
Ligand Receptor:
CD80 (B7-1), CD86 (B7-2)
Antigen References:
1. Barclay, A.N. et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Lenschow, D.J., et al., 1996. Annu. Rev. Immunol. 14:233. 3. Gross, J.A., et al., 1992. J. Immunol. 149:380.
