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The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101810).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB) 2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA) 3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA) 4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC) 5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF) 6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed 7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed 8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed 9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed 10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed 11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
C57BL/6 mouse splenocytes stained with M1/69 PE/Cy5
CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.
Other Names:
Heat Stable Antigen (HSA), Ly-52, Nectadrin
Structure:
GPI-linked sialoglycoprotein, 35-45 kD
Distribution:
B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells
Function:
Regulates B cell proliferation and differentiation
Ligand Receptor:
P-selectin, CD24
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Aigner S, et al. 1997. Blood 89:3385. 3. Hough MR, et al. 1996. J. Immunol. 156:479. 4. Liu Y, et al. 1992. J. Exp. Med. 175:437.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD24
C57BL/6 splenocytes stained with biotinylated M1/69, followed by Sav-PE
FITC anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 FITC
LEAF™ Purified anti-mouse CD24
C57BL/6 mouse splenocytes stained with LEAF™ purified M1/69, followed by anti-rat IgG FITC
PE anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 PE
Purified anti-mouse CD24
C57BL/6 mouse splenocytes stained with purified M1/69, followed by anti-rat IgG FITC
PE/Cy5 anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 PE/Cy5
APC anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 APC
Alexa Fluor® 488 anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 Pacific Blue™ M1/69
PE/Cy7 anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 PE/Cy7
PerCP/Cy5.5 anti-mouse CD24
C57 splenocytes stained with M1/69 PerCP/Cy5.5
Brilliant Violet 421™ anti-mouse CD24
C57BL/6 splenocytes were stained with CD24 (clone M1/69) Brilliant Violet 421™ (filled histogram) or with rat IgG2b Brilliant Violet 421™ isotype control (open histogram).
