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The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Ohl L, et al. 2004. Immunity 21:279. 2. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472. 3. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC) 4. Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed 5. Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed 6. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) 7. Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC) 8. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) 9. Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed 10. Kang SG, et al. 2007. J. Immunol. 179:3724. 11. Mao A et al. 2005. J. Immunol. 175:5146. PubMed 12. Allende ML, et al. 2008. FASEB J. 22:307. PubMed 13. Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed 14. Chen H, et al. 2005. J. Immunol. 175:591. PubMed 15. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Balb/c splenocytes stained with CD3 APC and 4B12 PE/Cy5
CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.
Other Names:
C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
Hematopoietic stem cells, T subsets, mature dendritic cells
Function:
Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
Ligand Receptor:
MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
Antigen References:
1. Schweickart VL, et al. 1994. Genomics 23:643. 2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118. 3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053. 4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD197 (CCR7)
Biotin anti-mouse CD197 (CCR7)
C57BL/6 mouse splenocytes stained with CD3 FITC and biotinylated 4B12 (top) or biotinylated rat IgG2a, k isotype control (bottom), followed by Sav-PE
PE anti-mouse CD197 (CCR7)
C57BL/6 splenocytes stained with CCR7-PE (clone 4B12) and CD44-FITC (gated on CD3+ cell population)
C57BL/6 splenocytes stained with rat IgG2a-PE (control) and CD44-FITC (gated on CD3+ cell population)
APC anti-mouse CD197 (CCR7)
C57BL/6 splenocytes stained with 4B12 APC and 145-2C11 (CD3) PE
Alexa Fluor® 647 anti-mouse CD197 (CCR7)
C57BL/6 Splenocytes stained with RIgG2a, κ Alexa Fluor® 647 (top) or 4B12 Alexa Fluor® 647 (bottom) and CD3 PE.
Alexa Fluor® 488 anti-mouse CD197 (CCR7)
C57BL/6 splenocytes stained with 4B12 Alexa Fluor® 488 and CD3 (145-2C11) PE
PE/Cy5 anti-mouse CD197 (CCR7)
Balb/c splenocytes stained with CD3 APC and 4B12 PE/Cy5
PerCP/Cy5.5 anti-mouse CD197 (CCR7)
C57BL/6 mouse splenocytes stained with CD3 (17A2) FITC and 4B12 PerCP/Cy5.5
Brilliant Violet 421™ anti-mouse CD197 (CCR7)
C57BL/6 splenocytes were stained with CD3 FITC and CD197 (clone 4B12) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ (bottom).
