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The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Granucci F, et al. 1997. J. Immunol. 159:1794. 2. Stokes RW, et al. 1998. J. Immunol. 160:5514. 3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP) 4. Ma XT, et al. 2006. Cancer Research 66:1169. 5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF) 6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed 7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed 8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed 9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed 10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed 11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed 12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed 13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed 14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed 15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed 16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed 17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE/Cy5 N418 (top) or PE/Cy5 Armenian hamster IgG isotype control (bottom)
CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.
Other Names:
αX integrin, integrin αX chain, CR4, p150
Structure:
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution:
dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function:
Cellular adhesion
Ligand Receptor:
iC3b, fibrinogen
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press. 2. Springer TA. 1994. Cell 76:301. 3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 APC and PK136 PE
Biotin anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and biotinylated N418 (top) or biotinylated Armenian hamster IgG isotype control (bottom), followed by Sav-PE
FITC anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
PE anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE N418 (top) or PE Armenian hamster IgG isotype control (bottom)
Purified anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC
Alexa Fluor® 488 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and Alexa Fluor® 488 N418 (top) or Alexa Fluor® 488 Armenian hamster IgG isotype control (bottom)
Alexa Fluor® 647 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647
PE/Cy5 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE/Cy5 N418 (top) or PE/Cy5 Armenian hamster IgG isotype control (bottom)
PE/Cy7 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE/Cy7 N418 (top) or PE/Cy7 Armenian hamster IgG isotype control (bottom)
Brilliant Violet 605™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 605™.
Alexa Fluor® 700 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 700 and M5/114.15.2 PE
Pacific Blue™ anti-mouse CD11c
C57BL/6 mouse splenocytes stained with Pacific Blue™ N418
APC/Cy7 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cy7 (top) or Armenian hamster IgG APC/Cy7 isotype control (bottom).
PerCP/Cy5.5 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 PerCP/Cy5.5
PerCP anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 PerCP and M5/114.15.2 PE
Brilliant Violet 421™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 570™ (top) or Armenian hamster IgG Brilliant Violet 570™ isotype control (bottom).
