The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining4 of acetone-fixed frozen tissue sections.
Application References:
1. Knapp, W., et al., 1989. Leucocyte Typing IV. Oxford University Press, New York. 2. Zhou, L., et al., 1995. J. Immunol. 154:3821. 3. Cao, W., et al., 2005. Biochem. J. 385:85. 4. Lore, K., et al., 2002. AIDS 16:683. 5. Cho, H., et al., 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006
Monocyte-derived dendritic cells (induced with GM-CSF+IL-4+TNF-?) stained with HB15e PE/Cy5
Description:
CD83 is a 43 kD single chain type I glycoprotein also known as HB15. A member of the immunoglobulin superfamily, CD83 is expressed on a subset of dendritic cells, Langerhans cells, and weakly on activated lymphocytes. Although CD83 is thought to play a role in antigen presentation and/or lymphocyte activation, the precise function of this protein is unknown. CD83 is considered to be a useful marker for mature dendritic cells.
Other Names:
HB15
Structure:
Ig superfamily, single chain transmembrane glycoprotein, 43 kD
Distribution:
Dendritic cells, Langerhan cells, activated B and T cells
Antigen References:
1. Kozlow, E., et al., 1993. Blood 81:454. 2. Zhou, L., et al., 1992. J. Immunol. 149:735. 3. Zhou, L., et al., 1995. Blood 86:3295.
