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The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections, and blocking of T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 317404).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. 3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed 4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed 5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Human peripheral blood lymphocytes stained with OKT4 PE/CY5
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding.
Other Names:
T4
Structure:
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution:
T cell subset, majority of thymocytes, monocytes/macrophages
Function:
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand Receptor:
MHC class II molecules, HIV gp120, IL-16
Antigen References:
1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD4
Human peripheral blood lymphocytes stained with purified OKT4, followed by anti-mouse IgGs FITC
LEAF™ Purified anti-human CD4
Human peripheral blood lymphocytes stained with LEAF™ purified OKT4, followed by anti-mouse IgGs FITC
Biotin anti-human CD4
Human peripheral blood lymphocytes stained with biotinylated OKT4, followed by Sav-PE
FITC anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 FITC
PE anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 PE
PE/Cy5 anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 PE/CY5
PE/Cy7 anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 PE/CY7
APC anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 APC
APC/Cy7 anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 APC/Cy7
Alexa Fluor® 488 anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 Alexa Fluor® 647
Alexa Fluor® 700 anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 Alexa Fluor® 700
Pacific Blue™ anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 Pacific Blue™
PerCP/Cy5.5 anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 PerCP/Cy5.5
PerCP anti-human CD4
Human peripheral blood lymphocytes stained with OKT4 PerCP
