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The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.
Other Names:
Glycophorin A/B, GPA/GPB
Structure:
Sialoglycoprotein, 20 kD
Distribution:
Erythrocytes
Antigen References:
1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD235ab
Human red blood cells stained with HIR2 APC
PE anti-human CD235ab
Human red blood cells stained with
CD235a/b PE
PE/Cy5 anti-human CD235ab
Human red blood cells stained with HIR2 PE/Cy5
Purified anti-human CD235ab
Human red blood cells stained with
CD235a/b PE
FITC anti-human CD235ab
Human peripheral red blood cells stained with HIR2 FITC
Pacific Blue™ anti-human CD235ab
Human erythrocytes stained with HIR2 Pacific Blue™
PerCP/Cy5.5 anti-human CD235ab
Human red blood cells were stained with anti-human CD235ab (clone HIR2) PerCP/Cy5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).
