The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: The 5E8/CXCR2 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR2 expression.
Human peripheral blood granulocytes stained with 5E8/CXCR2 PE/Cy5
Description:
CXCR2 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor B (IL-8RB), CD182, and CD128b. It is a CXC chemokine receptor belongs to G protein-coupled receptor (CPCR) family. CXCR2 is expressed as homodimer or heterodimer with CXCR1 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. CXCR2 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α,β,γ (CXCL1, CXCL2, CXCL3).
Other Names:
CD182, CXCR2, CD128b, IL-8RB, Type II IL-8 receptor
Structure:
Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR1
Distribution:
Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes
1. Chuntharapai, A., et al., 1994. J. Immunol. 153:5682. 2. Wilson, S., et al., 2005. J. Biol. Chem. 280:28663. 3. Emadi, S., et al., 2005. Blood 105:464. 4. Omari, K. M., et al., 2005. Brain 128:1003. 5. Juremalm, M., Nilsson., G., 2005. Chem. Immunol. Allergy 87:130. 6. Wolf, M., et al., 1998. Eur. J. Immunol. 28:164.
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