The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: The 8F1/CXCR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR1 expression.
Human peripheral blood granulocytes stained with 8F1/CXCR1 PE/Cy5
Description:
CXCR1 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor A (IL-8RA), CD181, and CDw128a. It is a CXC chemokine receptor belongs to G protein-coupled receptor (CPCR) family. CXCR1 is expressed as homodimer or heterodimer with CXCR2 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. The expression level of CXCR1 on monocytes is lower that of CXCR2. CXCR1 mediates nurophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α(CXCL1).
Other Names:
CD181, CXCR1, CDw128, CD128a, IL-8RA, Type I IL-8 receptor
Structure:
Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR2
Distribution:
Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes
1. Chuntharapai, A., et al., 1994. J. Immunol. 153: 5682. 2. Wilson, S., et al., 2005. J. Biol. Chem. 280: 28663. 3. Emadi, S., et al., 2005. Blood 105: 464. 4. Omari, K. M., et al., 2005. Brain 128: 1003. 5. Juremalm, M., G. Nilsson. 2005. Chem. Immunol. Allergy. 87: 130. 6. Wolf, M., et al., 1998. Eur. J. Immunol. 28: 164
