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The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Human pre-B cell line REH stained with BV10A4H2 PE/Cy5
CD135 is a 130-160 type III tyrosine kinase receptor expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL and CML-BC. CD135, also known as FMS-like tyrosine kinase-3, FLT3, STK-1, and Flk-2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc.
Other Names:
FMS-like tyrosine kinase-3, FLT-3, STK-1, Flk-2
Structure:
Member of the immunoglobulin supergene family, type 3 tyrosine kinase receptor; 130-160 kD
Distribution:
Expressed on CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. Also expressed on AML, ALL and CML-BC tumor cells
Function:
Tyrosine kinase growth factor receptor involved in the growth and differentiation of primitive hematopoietic cells
Ligand Receptor:
FLT3 ligand
Antigen References:
1. Rappold I, et al. 1997. Blood 90:111. 2. Rosnet O, et al. 1996. Acta Haematol. 95:218. 3. Rosnet O, et al. 1996. Leukemia 10:238. 4. Bertho JM, et al. 2000. Scand. J. Immunol. 52:53.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD135 (Flt-3/Flk-2)
Human pre-B leukemia cell line REH stained with purified BV10A4H2, followed by biotinylated anti-mouse IgG and Sav-PE
PE anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH stained with BV10A4H2 PE
APC anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH stained with BV10A4H2 APC
PE/Cy5 anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH stained with BV10A4H2 PE/Cy5
Biotin anti-human CD135 (Flt-3/Flk-2)
Human pre-B cell line REH stained with biotinylated BV10A4H2, followed by Sav-PE
