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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Fox CC, et al. 1994. Cell Immunol. 158(1):253 2. Chen-Woan M, et al. 1996. J. Leukocyte Biol. 59:196 3. Damoiseaux JG, et al. 1998. J. Leukocyte Biol. 64(6):803
LOU rat splenocytes stained with 1F4 (CD3) Alexa Fluor® 647 and OX-6 PE
RT1B is expressed on peripheral B lymphocytes, epidermal Langerhans cells, dendritic cells, some tissue macrophages, subsets of thymocytes, peritoneal mast cells, and small intestinal villus epithelium. The OX-6 antibody reacts with non-polymorphic determinants of the rat MHC class II antigen, I-A equivalent. The OX-6 monoclonal antibody cross-reacts with mouse I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A [g7]) mice.
Other Names:
MHC-class II, I-A equivalent
Structure:
MHC-class II
Distribution:
B lymphocytes, epidermal Langerhans cells, dendritic cells, some tissue macrophage, and subsets of thymocytes.
PE Anti-rat RT1B, OX-6, PE, MHC-class II, I-A equivalent, Rat, Cross-Reactivity: Cross-reacts with mouse I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A [g7]) mice., Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-rat RT1B
LOU rat splenocytes stained with anti-rat CD3 (1F4) APC and purified OX-6, followed by anti-mIgG1-FITC
FITC anti-rat RT1B
LOU rat splenocytes stained with OX-6 FITC and CD3 APC
PE Anti-rat RT1B
LOU rat splenocytes stained with 1F4 (CD3) Alexa Fluor® 647 and OX-6 PE
