Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References:
1. Lin CH and Hunig T, et al. 2003. Eur. J. Immunol. 33:626. (IP) 2. Nessi V, et al. 2010. J. Immunol. 185:5656. PubMed
LOU rat lymph node cells were stimulated with plate-bound CD3 and CD28 for 3 days, then surface stained with CD4 PerCP. This was followed by fixation and permeabilization with BioLegend's FOXP3 Fix/Perm Buffer Set and intracellular staining with FOXP3 Alexa Fluor® 647, and CD152 (clone WKH203) PE (top) or mouse IgG1, κ PE isotype control (bottom). The data was analyzed by gating on CD4-positive cells.
CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation.
Other Names:
Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Structure:
Ig superfamily, 33 kD
Distribution:
Activated T and B cells
Function:
Negative regulator of T cell activation
Ligand Receptor:
CD80 (B7-1), CD86 (B7-2)
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Allison JP, et al. 1995. Science 270:932. 3. Waterhouse P, et al. 1995. Science 270:985. 4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-rat CD152
LOU rat lymph node cells were stimulated with plate-bound CD3 and CD28 for 3 days, then surface stained with CD4 PerCP. This was followed by fixation and permeabilization with BioLegend's FOXP3 Fix/Perm Buffer Set and intracellular staining with FOXP3 Alexa Fluor® 647, and CD152 (clone WKH203) PE (top) or mouse IgG1, κ PE isotype control (bottom). The data was analyzed by gating on CD4-positive cells.
