The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC-quality Tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 109308).
Application References:
1. Kayagaki, N., et al. 1999. J. Immunol. 163:1906. 2. Sato, K., et al., 2005. J. Immunol. 174:4025. 3. Joshi, P.S., et al., 2006. J. Leukoc. Biol. 80:1345. 4. Herold, S., et al. 2008. J. Exp Med. PubMed 5. Iannello, A., et al. 2009. J. Virol. 83:5999. PubMed
Mouse TRAIL transfected cells stained with N2B2 PE
Description:
CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicity in vitro.
