The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry2 of acetone-fixed frozen sections, and in vivo depletion of γ/δ T cells3-5.
Application References:
1. Goodman, T., et al., 1989. J. Exp. Med. 170: 1569. 2. Cardon, A.E., et al., 2003. Infect. Immun. 71:2634. 3. Kapp, J.A., et al., 2004. Immunology 111:155. 4. Skelsey, M.E., et al., 2001. J. Immunol. 166:4327. 5. Ke, Y., et al., 1997. J. Immunol. 158:3610. 6. Podd, B.S., et al., 2006. J. Immunol. 176: 6532. 7. Akilov, OE., et al. 2009. J. Immunol. 183:7994. PubMed.
C57BL/6 splenocytes stained with CD3 (145-2C11) APC and GL3 PE
Description:
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. The GL3 antibody has been shown to be useful to identify γ/δ T cells by flow cytometry and immunohistochemistry and to deplete γ/δ T cells in vivo.
Other Names:
T cell receptor gamma/delta
Structure:
Ig superfamily, associates with CD3 complex
Distribution:
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
Function:
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand Receptor:
Some bacterial or tumor antigens bound to MHC class I.
Receptors:
Some bacterial or tumor antigens bound to MHC class I
Antigen References:
1. Skarstein, K., et al., 1995. Immunology 81: 497. 2. Harrison, L.C., et al., 1996. J. Exp. Med. 184:2167. 3. Wildner, G., et al., 1996. Eur. J. Immunol. 26: 2140. 4. Brandes, M., et al., 2005. Science 309: 264.
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