The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC1
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry2 of acetone-fixed frozen sections, and in vivo depletion of γ/δ T cells3-5.
Application References:
1. Goodman, T., et al., 1989. J. Exp. Med. 170: 1569. 2. Cardon, A.E., et al., 2003. Infect. Immun. 71:2634. 3. Kapp, J.A., et al., 2004. Immunology 111:155. 4. Skelsey, M.E., et al., 2001. J. Immunol. 166:4327. 5. Ke, Y., et al., 1997. J. Immunol. 158:3610. 6. Podd, B.S., et al., 2006. J. Immunol. 176: 6532. 7. Akilov, OE., et al. 2009. J. Immunol. 183:7994. PubMed.
C57BL/6 splenocytes stained with CD3 (145-2C11) APC and GL3 PE
Description:
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. The GL3 antibody has been shown to be useful to identify γ/δ T cells by flow cytometry and immunohistochemistry and to deplete γ/δ T cells in vivo.
Other Names:
T cell receptor gamma/delta
Structure:
Ig superfamily, associates with CD3 complex
Distribution:
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
Function:
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand Receptor:
Some bacterial or tumor antigens bound to MHC class I.
Receptors:
Some bacterial or tumor antigens bound to MHC class I
Antigen References:
1. Skarstein, K., et al., 1995. Immunology 81: 497. 2. Harrison, L.C., et al., 1996. J. Exp. Med. 184:2167. 3. Wildner, G., et al., 1996. Eur. J. Immunol. 26: 2140. 4. Brandes, M., et al., 2005. Science 309: 264.
