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PE anti-mouse TCR β chain Antibody
Cat. # Size Price  

109207 50 µg $60.00
    
109208 200 µg $180.00
    
Product Details

Clone: H57-597 (See other available formats)
Isotype: Armenian Hamster IgG
Isotype Control:PE Armenian Hamster IgG Isotype Ctrl
Reactivity: Mouse
Immunogen: Affinity purified TCR from mouse DO-11.10 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation
Laser:
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application
Notes:
H57-597 is a hamster mAb directed to an epitope of the C region of TCR β chain.12 The H57-597 antibody does not cross-react with γ/δ TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate α/β TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 109214).
Application
References:
  Publication Library
 Image 1
C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ (UC7-13D5) FITC


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See PE spectral data





 


Antigen Details

Description: T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.
Other Names: TCR-β chain, TCR-β, β-TCR
Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution: Majority of T cells and thymocytes (correlated to differentiation)
Function: Antigen recognition, T cell activation
Ligand Receptor: Peptide bound-MHC class I and II
Antigen
References:
1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.
GeneID: 21577
UniProt: View information about TCR beta chain on UniProt.org
Keywords: PE anti-mouse TCR β chain, H57-597, PE, TCR-β chain, TCR-β, β-TCR, Mouse, Flow Cytometry, Immunology, Antibodies
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Cell Surface Immunofluorescence Staining Protocol
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Version: 2 Revision Date: 2014-02-06
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse TCR β chain
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with H57-597 APC

  • Biotin anti-mouse TCR β chain
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with biotinylated H57-597, followed by Sav-PE

  • FITC anti-mouse TCR β chain
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with H57-597 FITC

  • LEAF™ Purified anti-mouse TCR β chain
    C57BL/6 CD3+ splenocytes stained with

    C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ (UC7-13D5) FITC

  • PE anti-mouse TCR β chain
    C57BL/6 CD3+ splenocytes stained with

    C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ (UC7-13D5) FITC

  • PE/Cy5 anti-mouse TCR β chain
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with H57-597 PE/Cy5.

  • Purified anti-mouse TCR β chain
    C57L/6 splenocytes stained with H57-597

    C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC

  • Alexa Fluor® 488 anti-mouse TCR β chain
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-mouse TCR β chain
    C57BL/6 splenocytes stained with H57-597

    C57BL/6 splenocytes stained with H57-597 Alexa Fluor® 647

  • APC/Cy7 anti-mouse TCR β chain
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) APC/Cy7 (filled histrogram) or Armenian hamster IgG APC/Cy7 isotype control (open histogram).

  • PE/Cy7 anti-mouse TCR β chain
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with H57-597 PE/Cy7

  • Alexa Fluor® 700 anti-mouse TCR β chain
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® 700

  • Pacific Blue™ anti-mouse TCR β chain
    C57BL/6 splenocytes stained with H57-597

    C57BL/6 splenocytes stained with H57-597 Pacific Blue™

  • Brilliant Violet 421™ anti-mouse TCR β chain
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 421™ (filled histrogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram).

  • PerCP/Cy5.5 anti-mouse TCR β chain
    C57BL/6 splenocytes stained with H57-597

    C57BL/6 splenocytes stained with H57-597 PerCP/Cy5.5

  • Brilliant Violet 570™ anti-mouse TCR β chain
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 570™ (filled histogram) or Armenian hamster IgG Brilliant Violet 570™ isotype control (open histogram).

  • Brilliant Violet 510™ anti-mouse TCR β chain
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 510™ (filled histrogram) or Armenian hamster IgG Brilliant Violet 510™ isotype control (open histogram).

  • Purified anti-mouse TCR β chain (MaxPar® Ready)
    Mouse splenocytes stained with 143Nd-anti-TCRβ

    Mouse splenocytes stained with 143Nd-anti-TCRβ (H57-597) and 144Nd-anti-B220 (CD45R) (RA3-6B2). Data provided by DVS Sciences.

  • Alexa Fluor® 594 anti-mouse TCR β chain
    C57BL/6 mouse frozen lymph node

    C57BL/6 mouse frozen lymph node sections were fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 2.5 µg/ml of TCR β chain (clone H57-597) Alexa Fluor® 594 (red) and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.

    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Alexa Fluor® 594 (filled histogram). The data was acquired by BD LSRFortessa™ cell analyzer equipped with Yellow-Green Laser (561 nm).

  • PE/Dazzle™ 594 anti-mouse TCR β chain
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with TCR-β (clone H57-597) PE/Dazzle™ 594 (filled histogram) or Armenian hamster IgG PE/Dazzle™ 594 isotype control (open histogram).

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