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PE anti-mouse Notch 4 Antibody
PE anti-mouse Notch 4 Antibody
128407 50 µg ¥30,000     
128408 200 µg ¥70,000     
Product Details
Clone: HMN4-14
Isotype: Armenian Hamster IgG
Isotype Control:PE Armenian Hamster IgG Isotype Ctrl
Reactivity: Mouse
Immunogen: Notch4-Fc recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
References:

1. Moriyama Y,et al.2008. Intl J. Immunol. 20:763.
2. Yan Q, et al. 2010. Am. J PAthol 176:2921. PubMed
FN4/CHO (mouse Notch-4 transfected) cells
FN4/CHO (mouse Notch-4 transfected) cells stained with HMN4-14 PE


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See PE spectral data



Description:

The Notch receptors and their ligands are highly conserved from invertebrates to mammals.The Delta-like 1 (Dll1) is one of the four or five Notch ligands identified.The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus.This translocation triggers a series of signaling process.Delta-like 1 is reported to be essential for the maintenance of marginal zone B cells in normal mice and engagement of Notch 1 by Dll 1 promotes differentiation of B lymphocytes to antibody-secreting cells.
Other Names: Proto-oncogene of int-3
Structure: Type 1 transmembrane receptor with a large extracellular domain consisting of 29 -36 EGF( epidermal growth factor) and three LNR (Lin12-Notch) repeats.
Distribution: Notch 4 is preferentially expressed in endothelial cells.
Function: Regulation of cell development, including myogenesis, neurogenesis, and lymphocyte development. Notch 4 seems to play a crucial role in vasculogenesis and angiogenesis.
Ligand Receptor: Jagged 1, Jagged2, Delta-like 1, Delta-like3 and Delta-like 4
Antigen
References:

1.Ehebauer MT,et al. 2006. Biochem J.392:13.
2.Shimizu K,et al. 2000Mol Cell Biology. 20:18
3. Tanigaki K,et al.2007. Nature immunol. 8:451.
GeneID: 18132
Latest Publications: View the latest Notch 4 articles on HighwirePress.com
UniProt: View information about Notch 4 on UniProt.org
Keywords: PE anti-mouse Notch 4, HMN4-14, PE, Proto-oncogene of int-3 , Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse Notch 4
    FN4/CHO cells (mouse Notch-4 transfected

    FN4/CHO cells (mouse Notch-4 transfected cells) stained with purified HMN4-14, followed by anti-Armenian hamster IgG PE

  • PE anti-mouse Notch 4
    FN4/CHO (mouse Notch-4 transfected) cells

    FN4/CHO (mouse Notch-4 transfected) cells stained with HMN4-14 PE

  • Alexa Fluor® 647 anti-mouse Notch 4
    FN4/CHO (mouse Notch-4) transfected cell

    FN4/CHO (mouse Notch-4) transfected cell line stained with HMN4-14 Alexa Fluor® 647

  • APC anti-mouse Notch 4
    FN4/CHO (mouse Notch-4) transfected cell

    FN4/CHO (mouse Notch-4) transfected cell line stained with HMN4-14 APC

  • Biotin anti-mouse Notch 4
    Mouse Notch 4 transfected CHO

    Mouse Notch 4 transfected CHO cells were stained with biotinylated Notch 4 (clone HMN4-14) (filled histogram) or Armenian hamster IgG(open histogram), followed by Sav-APC.

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