Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Moriyama Y, et al. 2008. Int. Immunol. 20:763.
Mouse NOTCH-1 transfected cells stained with HMN1-12 PE
C57BL/6 mouse CD4 and CD8 double-negative thymocytes were stained with CD24 APC and Notch1 (clone HMN1-12) PE (top) or Armenian hamster IgG PE isotype control (bottom).
The Notch receptors are highly conserved from invertebrates to mammals. Notch 1 and Notch 2 exhibit the greatest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. Notch 3 is primarily expressed in adult arterial smooth muscle cells. Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.
Other Names:
Neurogenic locus notch homolog protein 1
Structure:
Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution:
Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.
Function:
Regulates broad spectrum of various cell lineage commitment processes, including myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Ligand Receptor:
Jag 1,2; Delta 1,2
Antigen References:
1. Ehebauer ME, et al. 2006. Biochem. J. 392:13. 2. Shimizu K, et al. 2000. Mol. Cell. Biol. 20:18. 3. Tanigaki K, et al. 2007. Nature Immunol. 8:4514. 4. Kraman M, et al. 2005. FASEB J. 19:1311.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Notch 1
Mouse Notch-1 transfected cells stained with purified HMN1-12, followed by anti-Armenian hamster IgG PE
LEAF™ Purified anti-mouse Notch 1
Mouse Notch-1 transfected cells stained with LEAF™ purified HMN1-12, followed by anti-Armenian hamster IgG PE
PE anti-mouse Notch 1
Mouse NOTCH-1 transfected cells stained with HMN1-12 PE
C57BL/6 mouse CD4 and CD8 double-negative thymocytes were stained with CD24 APC and Notch1 (clone HMN1-12) PE (top) or Armenian hamster IgG PE isotype control (bottom).
Alexa Fluor® 647 anti-mouse Notch 1
Mouse NOTCH-1 transfected cells stained with HMN1-12 Alexa Fluor® 647
Biotin anti-mouse Notch 1
Mouse Notch 1 transfected CHO cells were stained with biotinylated anti-Notch 1 (clone HMN1-12) (filled histogram) or Armenian Hamster IgG (open histogram), followed by Sav-APC.
APC anti-mouse Notch 1
C57BL/6 mouse CD4 and CD8 double-negative thymocytes were stained with CD24 PE and Notch1 (HMN1-12) APC (top) or Armenian hamster IgG APC isotype control (bottom).
