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PE anti-mouse Ly-49A Antibody
PE anti-mouse Ly-49A Antibody
116808 200 µg ¥56,000     
Product Details
Clone: YE1/48.10.6
Isotype: Rat IgG2a, κ
Isotype Control:PE Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Con A activated splenic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2

.
Application
References:

1. Takei F. 1983. J. Immunol. 130:2794.
2. Chan P, et al. 1989. J. Immunol. 142:1727.
C57BL/6 mouse splenocytes stained with
C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC


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See PE spectral data



Description:

Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2Dd and H-2Dk, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The YE1/48.10.6 antibody reacts with NK cells from all mouse strains tested and reverses the inhibition of NK cytotoxicity.
Structure: C-type lectin, Ly-49, ITIM, 44 kD
Distribution: NK and NK-T cells
Function: Inhibitory NK cell receptor
Ligand Receptor: MHC Class I Molecules
Antigen
References:

1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
GeneID: 16627
Latest Publications: View the latest Ly-49A articles on HighwirePress.com
UniProt: View information about Ly-49A on UniProt.org
Keywords: PE anti-mouse Ly-49A, YE1/48.10.6, PE, Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Biotin anti-mouse Ly-49A
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC

  • FITC anti-mouse Ly-49A
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC

  • PE anti-mouse Ly-49A
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC

  • Purified anti-mouse Ly-49A
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with YE1/48.10.6 PE and DX5 FITC

  • Pacific Blue™ anti-mouse Ly-49A
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with anti-Ly49G FITC and YE1/48.10.6 Pacific Blue™

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