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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking1-4 of cell adhesion, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining5 of frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 120604).
Application References:
1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) 2. Berlin C, et al. 1993. Cell 74:185. (Block) 3. Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block) 4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block IP) 5. Bogetto L, et al. 2000. Blood 95:2397. (IHC)
C57BL/6 mouse splenocytes stained with DATK32 PE
C57/B6 mouse bone marrow cells were surface stained with B220 APC and LPAM-1 PE (clone DATK32) (top) or rat IgG2a, κ PE isotype control (bottom).
DATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d/β7, or LPAM-1. It belongs to Ig superfamily found on majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and helps direct the migration of blood lymphocytes to the intestine and associated lymphoid tissues. It has been reported that the DATK32 mAb is able to in vitro and in vivo block α4β7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1 and fibronectin
Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells
Function:
Lymphocyte adhesion
Ligand Receptor:
VCAM-1 (CD106), MAdCAM-1, fibronectin
Antigen References:
1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Berlin C, et al. 1994. Cell 74:185. 3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243. 4. Hamann A, et al. 1994. J. Immunol. 152:3282.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse LPAM-1 (Integrin α4β7)
C57BL/6 mouse splenocytes stained with purified DATK32, followed by anti-rat IgG FITC
PE anti-mouse LPAM-1 (Integrin α4β7)
C57BL/6 mouse splenocytes stained with DATK32 PE
C57/B6 mouse bone marrow cells were surface stained with B220 APC and LPAM-1 PE (clone DATK32) (top) or rat IgG2a, κ PE isotype control (bottom).
APC anti-mouse LPAM-1 (Integrin α4β7)
C57BL/6 splenocytes stained with DATK32 APC
C57BL/6 mouse bone marrow cells were surface stained with B220 PE and LPAM-1 APC (clone DATK32) (top) or rat IgG2a, κ APC isotype control (bottom).
