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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Clone MM27-7B1 specifically recognizes the p28 subunit of IL-27.1
Application References:
1. Uyttenhove C, et al. 2011. J. Leukoc. Biol. 89:1001.
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) Alexa Fluor® 647 and MM27-7B1 PE
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) Alexa Fluor® 647 and mouse IgG2a PE isotype control
Interleukin IL-27 is a heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 P40-related protein) and P28 (a newly discovered IL-12 P35-related protein). It is a member of the IL-6/IL-12 cytokine family and mainly produced by antigen-presenting cells, including macrophages and dendritic cells. IL-27 acts on T cells and NK cells. It has been reported that IL-27 drives rapid clonal expansion of naïve CD4+ T cells, promotes Th1 polarization, and IFN-γ production in synergy with IL-12. The IL-27 induced Th1 differentiation was mediated by rapid and marked up-regulation of ICAM-1/LFA-1 interaction in a STAT1-dependent manner. IL-27 has an anti-inflammatory function by enhancing Th1 cell differentiation, a potent antitumor activity, through CD8+ T cell and NK cell activation and a potential therapeutic role for autoimmune disease by inhibiting Th-17 development. IL-27 mediates its biological effects through its receptor, WSX-1/T cell cytokine receptor (TCCR), which is homologous to the IL-12Rβ2 subunit. Protein gp130 serves as a functional signal-transducing molecule for IL-27.
Other Names:
Interleukin-27 p28, IL-27-A, IL-27 subunit alpha
Structure:
A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 P40-ralated protein) and P28 (a newly discovered IL-12 P35-related protein).
Distribution:
Produced by antigen-presenting cells, including macrophages and dendritic cells.
Function:
Drive rapid clonal expansion of naïve CD4+ T cells, promote Th1 polarization and IFN-γ production; play roles in anti-inflammation, antitumor, and potential autoimmune disease therapy.
Ligand Receptor:
IL-27R (TCCR/WSX-1)
Antigen References:
1. Salcedo R, et al. 2004. J. Immunol. 173:7170. 2. Owaki T, et al. 2005. J. Immunol. 175:2191. 3. Pflanz S, et al. 2002. Immunity 16:779. 4. Diveu C, et al. 2009. J. Immunol. 182:5748. 5. Morishima N, et al. 2005. J. Immunol. 175:1686. 6. Liu J, et al. 2007. J. Exp. Med. 204:141.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 647 anti-mouse IL-27 p28
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) PE and MM27-7B1 Alexa Fluor® 647 (top) or mouse IgG2a Alexa Fluor® 647 isotype control (bottom).
APC anti-mouse IL-27 p28
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (M3/84) PE and IL-27 (MM27-7B1) APC
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (M3/84) PE and mouse IgG2a APC isotype control
PE anti-mouse IL-27 p28
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) Alexa Fluor® 647 and MM27-7B1 PE
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) Alexa Fluor® 647 and mouse IgG2a PE isotype control
PE/Cy7 anti-mouse IL-27 p28
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) Alexa Fluor® 647 and MM27-7B1 PE/Cy7
Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (Mac-3) Alexa Fluor® 647 and mouse IgG2a PE/Cy7 isotype control
