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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Clone M3/38 has been reported to recognize residues 48–100 in the amino-terminal domain of galectin-3.7
Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.
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Purified anti-mouse/human Mac-2 (Galectin-3)
BALB/c peritoneal macrophages stained with M3/38 purified, followed by anti-rat IgG FITC
Biotin anti-mouse/human Mac-2 (Galectin-3)
BALB/c peritoneal marcophages stained with M3/38 biotin, followed by Sav-PE
PE anti-mouse/human Mac-2 (Galectin-3)
BALB/c peritoneal marcophages stained with M3/38 PE
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with CD107b Alexa Fluor® 647 and Mac-2 (clone M3/38) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).