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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser:
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1.
The 15-5-5 antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.
Other Names:
MHC class I
Structure:
MHC class I
Distribution:
Nucleated cells of the H-2Dk haplotype
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD8
Antigen References:
1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
PE anti-mouse H-2Dk, 15-5-5, PE, MHC class I, Mouse k haplotype, Cross-reacts with H-2Kd and H-2f haplotypes. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s)., Flow Cytometry, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse H-2Dk
C3H/He mouse splenocytes were stained with H-2Dk (clone 15-5-5) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
Purified anti-mouse H-2Dk
C3H/He mouse splenocytes were stained with purified H-2Dk (clone 15-5-5) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a FITC.
