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The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3.
Application References:
1. Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC) 2. Jameson J, et al. 2004. J. Immunol. 172:3573. (IF) 3. Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
C57BL/6 mouse splenocytes stained with CD90.2 (53-2.1) PE
CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.
Structure:
Ig superfamily, 25-35 kD
Distribution:
Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice
Function:
Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand Receptor:
CD45
Antigen References:
1. Borrello M, et al. 1996. Cell. Immunol.173:198. 2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220. 3 . Williams A, et al. 1982.Science 216:696
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD90.2 (Thy-1.2)
C57BL/6 mouse thymocytes stained with purified CD90.2 (53-2.1) conjugated with FITC
FITC anti-mouse CD90.2 (Thy-1.2)
C57BL/6 mouse thymocytes stained with CD90.2 (53-2.1) FITC
Pacific Blue™ anti-mouse CD90.2 (Thy-1.2)
C57BL/6 mouse thymocytes stained with CD90.2 (53-2.1) PB
Biotin anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse splenocytes stained with biotinylated CD90.2 (53-2.1), followed by Sav-PE (the FcR- mediated binding was blocked by pre-incubation with anti-mouse CD16/32, clone 93)
PerCP anti-mouse CD90.2 (Thy1.2)
C57BL/6 mouse splenocytes stained with CD90.2 (53-2.1) PerCP
PE anti-mouse CD90.2 (Thy-1.2)
C57BL/6 mouse splenocytes stained with CD90.2 (53-2.1) PE
PE/Cy7 anti-mouse CD90.2 (Thy-1.2)
C57BL/6 mouse splenocytes stained with CD90.2 (53-2.1) PE/Cy7
