The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Won W-J et al. 2002. Immunol 168:5605
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cy5.5 and MZ3 PE
Description:
CD9 is a surface glycoprotein of the tetraspannin family. It is expressed on a variety of cells, including nerve, muscle cells and many cells of hematopoietic origin. CD9 is found to participate in forming a large molecular cell complex with other member proteins, such as MHC class II, CD19, CD5 and other TM4SF molecules. It is reported that CD9 is a marker of marginal zone B cells, B1 cells and plasma cells. The diverse functions of CD9 may largely depend upon its associated molecules on different cells.
Other Names:
DRAP-27, MRP-1, p-24
Structure:
24 kd surface glycoprotein belonging to the tetraspanin (TM4SF)family which is characterized by four transmembrane-spanning domains and two extracellular domains.
Distribution:
CD9 is expressed in nerve, muscle, keratinocytes, fibroblasts and a variety of hematopoietic cells including monocytes, macrophages, granulocytes , platelets and activated T and B cells.
Interaction:
CD9 may participate in forming large molecular complexes with other associated molecules.
Antigen References:
1. Boucheix C et al. 1991. J Biol Chem 266:117 2. Lanza F et al. 1991. J Biol Chem 266:10638
