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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Won WJ, et al. 2002. J. Immunol. 168:5605. (FC, WB)
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cy5.5 and MZ3 PE
CD9 is a surface glycoprotein of the tetraspannin family. It is expressed on a variety of cells, including nerve, muscle cells and many cells of hematopoietic origin. CD9 is found to participate in forming a large molecular cell complex with other member proteins, such as MHC class II, CD19, CD5 and other TM4SF molecules. It is reported that CD9 is a marker of marginal zone B cells, B1 cells and plasma cells. The diverse functions of CD9 may largely depend upon its associated molecules on different cells.
Other Names:
DRAP-27, MRP-1, p-24
Structure:
24 kd surface glycoprotein belonging to the tetraspanin (TM4SF)family which is characterized by four transmembrane-spanning domains and two extracellular domains.
Distribution:
CD9 is expressed in nerve, muscle, keratinocytes, fibroblasts and a variety of hematopoietic cells including monocytes, macrophages, granulocytes , platelets and activated T and B cells.
Interaction:
CD9 may participate in forming large molecular complexes with other associated molecules.
Antigen References:
1. Boucheix C, et al. 1991. J. Biol. Chem. 266:117 2. Lanza F, et al. 1991. J. Biol. Chem. 266:10638
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD9
C57BL/6 bone marrow cells double stained with MZ3 biotin (Lower panel) or rat IgG2a isotype control (Upper panel) followed by Sav-PE, and anti-mouse Ly-6G/Ly-6C (RB6-8C5) FITC.
PE anti-mouse CD9
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cy5.5 and MZ3 PE
FITC anti-mouse CD9
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cy5.5 and MZ3 FITC
Alexa Fluor® 647 anti-mouse CD9
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cy5.5 and MZ3 Alexa Fluor® 647
