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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 104710).
Application References:
1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC) 2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP) 3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block) 4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC) 5. Ma XT, et al. 2006. Cancer Res. 66:1169. 6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) 7. Lawson BR, et al. 2007. J. Immunol. 178:5366. 8. Turnquist HR, et al. 2007. J. Immunol. 178:7018. 9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily, also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Other Names:
B7-1, B7, Ly-53
Structure:
Ig superfamily, 60 kD
Distribution:
Macrophages, activated B and T cells, dendritic cells
Function:
T cell costimulation
Ligand Receptor:
CD28 (stimulatory), CD152(CTLA4)(inhibitory)
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD80
LPS-stimulated (3 days) BALB/c
splenocytes stained with 16-10A1
FITC
FITC anti-mouse CD80
LPS-stimulated (3 days) BALB/c
splenocytes stained with 16-10A1
FITC
LEAF™ Purified anti-mouse CD80
LPS-stimulated (3 days) BALB/c
splenocytes stained with 16-10A1
FITC
PE anti-mouse CD80
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
Purified anti-mouse CD80
LPS-stimulated (3 days) BALB/c
splenocytes stained with 16-10A1
FITC
PE/Cy5 anti-mouse CD80
LPS-stimulated (3 days) BALB/c
splenocytes stained with 16-10A1
FITC
APC anti-mouse CD80
LPS-stimulated (3 days) BALB/c
splenocytes stained with 16-10A1
FITC
