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Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isofrom and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition.
Other Names:
Decay accelerating factor (DAF)
Structure:
60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
Distribution:
Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.
Function:
Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
Antigen References:
1. Miwa T et al. 2001. Immunology 104:207 2. Lin FY et al. 2001 Immunology 104:215
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse CD55 (DAF)
C57BL/6 splenocytes stained with Riko-3 PE
Alexa Fluor® 647 anti-mouse CD55 (DAF)
C57BL/6 splenocytes stained with RA3-6B2 (B220) PE and Riko-3 Alexa Fluor®647